Fabrication of a hydrophobic/hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay
DC Field | Value | Language |
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dc.contributor.author | Lee, Moonkwon | - |
dc.contributor.author | Kim, Ki Hyung | - |
dc.contributor.author | Park, Jin-Goo | - |
dc.contributor.author | Lee, Jung-Hwan | - |
dc.contributor.author | Lim, Hyun-Woo | - |
dc.contributor.author | Park, Min-Yi | - |
dc.contributor.author | Chang, Soo-Ik | - |
dc.contributor.author | Lee, Eun Kyu | - |
dc.contributor.author | Lim, Dong Woo | - |
dc.contributor.author | Choo, Jaebum | - |
dc.date.accessioned | 2021-06-18T09:44:11Z | - |
dc.date.available | 2021-06-18T09:44:11Z | - |
dc.date.issued | 2012-03-20 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.issn | 2092-7843 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/46009 | - |
dc.description.abstract | In this work, we report on a simple process for fabricating a hydrophobic/hydrophilic hybrid-patterned microarray chip for a fast and sensitive immunoassay. Two different types of self-assembled monolayers (SAMs) were used in the fabrication of hydrophilic well patterns and hydrophobic substrates. The hydrophilic/hydrophobic hybrid SAM pattern generates a clear-cut boundary between the sample and the background. A change in the precursor molecules allows for many different. types of SAMs to be employed in the fabrication process. Fluorescence image-based detection has previously been used for the quantitative immune-analysis of a specific cancer marker. Here, a titanium-coated glass substrate was utilized to suppress auto-fluorescence signals from substrate backgrounds. Angiogenin (ANG), a small polypeptide implicated in both angiogenesis and tumor growth, was used as a target cancer marker for its validation. Assay results demonstrate that the hybrid-patterned array chip yields a narrower error deviation and a lower coefficient variation than in a conventional 96-well plate ELISA. Furthermore, the sample requirement (1 mu L) for the hybrid-patterned chip is about 50 times less than that required in an ELISA (at least 50 mu L). The proposed hydrophobic/hydrophilic hybrid-patterned microarray chip is expected to be a highly efficient tool that can be applied to a high throughput immunoassay of a specific cancer marker. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN BIOCHIP SOCIETY-KBCS | - |
dc.title | Fabrication of a hydrophobic/hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s13206-012-6102-y | - |
dc.identifier.bibliographicCitation | BIOCHIP JOURNAL, v.6, no.1, pp 10 - 16 | - |
dc.identifier.kciid | ART001642692 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000301684500002 | - |
dc.identifier.scopusid | 2-s2.0-84863339456 | - |
dc.citation.endPage | 16 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 10 | - |
dc.citation.title | BIOCHIP JOURNAL | - |
dc.citation.volume | 6 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Hydrophobic/hydrophilic pattern | - |
dc.subject.keywordAuthor | SAM | - |
dc.subject.keywordAuthor | Microarray | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordAuthor | Angiogenin | - |
dc.subject.keywordPlus | PROTEIN CHIPS | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | MICROSCOPY | - |
dc.subject.keywordPlus | ANGIOGENIN | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kciCandi | - |
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