염증세포주를 이용한 생체재료 용출물의 체외 생체적합성 평가
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신연호 | - |
dc.contributor.author | 송계용 | - |
dc.contributor.author | 서민지 | - |
dc.contributor.author | 김성민 | - |
dc.contributor.author | 박정극 | - |
dc.contributor.author | 김동섭 | - |
dc.contributor.author | 박기정 | - |
dc.contributor.author | 허찬회 | - |
dc.contributor.author | 차지훈 | - |
dc.contributor.author | 서영권 | - |
dc.date.accessioned | 2021-06-18T11:44:14Z | - |
dc.date.available | 2021-06-18T11:44:14Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/46593 | - |
dc.description.abstract | Various biometerials have been researched and have been developed for treatment of some disease through transplantation to body. They have been evaluated by in vitro cytotoxicity test using some skin-derived cell lines for prediction of their biocompatibility in vivo. However, the results of experiments using mesenchymal or epithelial cells could not be considered in vivo immune reaction. In this study, we evaluated the biomaterial-elution (elute from high density polyethylene film) using some cell lines (L929, Jurkat, U937) in vitro, and then that results were compared with in vivo results from guinea pig sensitization test. In sensitization test, saline and elution of syringe could not induce erythema, but only DNCB (hypersensitive chemical) induce erythema at guinea pig sensitization test. In cell experiment, the cytotoxicity results of inflammatory cells (Jurkat; T lymphocyte, U937; monocyte) was no difference with L929 (fibroblast) in the overall trend. However,inflammatory cell lines were only secreted inflammatory cytokine (TNF-α, INF-γ) in some materials (biomateriallution,FAC, DNCB). And the biomaterial-elution did not have toxicity to the cells, but it induced the inflammatory cytokines in inflammatory cell lines only. So, we were predicted inflammatory reaction through the cytokine resultes of inflammatory cell lines, and it was more correlated with in vivo results than cytotoxicity test. Therefore, we suggested that the inflammatory cytokine assay using inflammatory cell lines are more effective method in vitro for evaluation of biocompatibility of biomaterials or chemicals. | - |
dc.format.extent | 7 | - |
dc.publisher | 한국생물공학회 | - |
dc.title | 염증세포주를 이용한 생체재료 용출물의 체외 생체적합성 평가 | - |
dc.title.alternative | In vitro Biocompatibility Evaluation of Biomaterial-elution Using Inflammatory Cell Lines | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | KSBB Journal, v.26, no.3, pp 248 - 254 | - |
dc.identifier.kciid | ART001560602 | - |
dc.description.isOpenAccess | N | - |
dc.citation.endPage | 254 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 248 | - |
dc.citation.title | KSBB Journal | - |
dc.citation.volume | 26 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | inflammation | - |
dc.subject.keywordAuthor | biocompatibility | - |
dc.subject.keywordAuthor | inflammatory cell line | - |
dc.subject.keywordAuthor | fibroblast | - |
dc.subject.keywordAuthor | cytokine | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.