High sensitive detection of C-reactive protein by total internal reflection fluorescence microscopy on rapidly making nanoarray protein chip
DC Field | Value | Language |
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dc.contributor.author | Islam, Md. Shahinul | - |
dc.contributor.author | Lee, Hee Gu | - |
dc.contributor.author | Choo, Jaebum | - |
dc.contributor.author | Song, Joon Myong | - |
dc.contributor.author | Kang, Seong Ho | - |
dc.date.accessioned | 2021-06-18T12:43:55Z | - |
dc.date.available | 2021-06-18T12:43:55Z | - |
dc.date.issued | 2010-06-15 | - |
dc.identifier.issn | 0039-9140 | - |
dc.identifier.issn | 1873-3573 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/46859 | - |
dc.description.abstract | This study investigated a method for the high sensitivity detection and quantification of the C-reactive protein (CRP) in human serum using total internal reflection fluorescence microscopy (TIRFM) on a rapidly made nanoarray protein chip The nanoarray biotin-probe was patterned onto 3-mercaptopropyl trimethoxysilane-coated cover glass with a spot diameter of similar to 400 nm within 1 min using a NanoeNablei (TM)-based surface patterning tool The unlabeled CRP molecules were detected in human sera using TIRFM, based on a sandwich fluorescence immunoassay. The linear regression for standard CRP in the range of 50 zM-1 fM was determined using the equation y = 0 437x + 84 991 (R = 0.9993) This proposed method was similar to 2000 times faster than conventional atomic force microscopy based clip-pen nanolithography in terms of the chip manufacturing process Additionally this method was 6 x 10(6) times more sensitive than enzyme-linked immunosorbent assay and exhibited a wide dynamic linear range (50 zM-1 fM) (C) 2010 Elsevier B V All rights reserved | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | High sensitive detection of C-reactive protein by total internal reflection fluorescence microscopy on rapidly making nanoarray protein chip | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.talanta.2010.02.042 | - |
dc.identifier.bibliographicCitation | TALANTA, v.81, no.4-5, pp 1402 - 1408 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000278737100038 | - |
dc.identifier.scopusid | 2-s2.0-77952106895 | - |
dc.citation.endPage | 1408 | - |
dc.citation.number | 4-5 | - |
dc.citation.startPage | 1402 | - |
dc.citation.title | TALANTA | - |
dc.citation.volume | 81 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | C-reactive protein | - |
dc.subject.keywordAuthor | Nanoarray protein chip | - |
dc.subject.keywordAuthor | Surface patterning tool | - |
dc.subject.keywordAuthor | Single-molecule detection | - |
dc.subject.keywordAuthor | Total internal reflection fluorescence microscopy (TIRFM) | - |
dc.subject.keywordPlus | DIP-PEN NANOLITHOGRAPHY | - |
dc.subject.keywordPlus | DNA MICROARRAY | - |
dc.subject.keywordPlus | HUMAN SERUM | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | FILMS | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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