WNT/beta-catenin pathway modulates the TNF-alpha-induced inflammatory response in bronchial epithelial cells
DC Field | Value | Language |
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dc.contributor.author | Jang, Jaewoong | - |
dc.contributor.author | Jung, Yoonju | - |
dc.contributor.author | Chae, Seyeon | - |
dc.contributor.author | Chung, Sang-In | - |
dc.contributor.author | Kim, Seok-Min | - |
dc.contributor.author | Yoon, Yoosik | - |
dc.date.available | 2019-03-08T09:36:02Z | - |
dc.date.issued | 2017-03 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4695 | - |
dc.description.abstract | In this study, TNF-alpha was found to activate the WNT/beta-catenin pathway in BEAS-2B human bronchial epithelial cells. Levels of phospho-LRP6, Dv1-2, and phospho-GSK-3 beta were elevated, while that of Axin was reduced by TNF-alpha treatment. Nuclear translocation of beta-catenin and the reporter activity of a beta-catenin-responsive promoter were increased by TNF-alpha treatment. Under the same experimental conditions, TNF-alpha activated the NF-kappa B signaling, which includes the phosphorylation and degradation of I kappa B and nuclear translocation and target DNA binding of NF-kappa B, and it was found that an inhibitor of NF-kappa B activation, JSH-23, inhibited TNF-alpha-induced Wnt signaling as well as NF-kappa B signaling. It was also found that recombinant Wnt proteins induced NF-kappa B nuclear translocations and its target DNA binding, suggesting that Wnt signaling and NF-kappa B signaling were inter-connected. TNF-alpha-induced modulations of I kappa B and NE-kappa B as well as pro-inflammatory cytokine expression were significantly suppressed by the transfection of beta-catenin siRNA compared to that of control siRNA. Transfection of a beta-catenin expression plasmid augmented the TNF-alpha-induced modulations of I kappa B and NF-kappa B as well as pro-inflammatory cytokine expression. These results clearly demonstrated that the WNT/beta-catenin pathway modulates the inflammatory response induced by TNF-alpha, suggesting that this pathway may be a useful target for the effective treatment of bronchial inflammation. (C) 2017 Published by Elsevier Inc. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | WNT/beta-catenin pathway modulates the TNF-alpha-induced inflammatory response in bronchial epithelial cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2017.01.156 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.484, no.2, pp 442 - 449 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000394735400035 | - |
dc.identifier.scopusid | 2-s2.0-85011015030 | - |
dc.citation.endPage | 449 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 442 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 484 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | TNF-alpha | - |
dc.subject.keywordAuthor | WNT | - |
dc.subject.keywordAuthor | beta-catenin | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | Bronchial epithelial cell | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | INTERLEUKIN-6 | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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