Demethylation of Polymethoxyflavones by Human Gut Bacterium, Blautia sp MRG-PMF1
DC Field | Value | Language |
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dc.contributor.author | Burapan, Supawadee | - |
dc.contributor.author | Kim, Mihyang | - |
dc.contributor.author | Han, Jaehong | - |
dc.date.available | 2019-03-08T09:36:03Z | - |
dc.date.issued | 2017-03 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.issn | 1520-5118 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4697 | - |
dc.description.abstract | Polymethoxyflavones (PMFs) were biotransformed to various demethylated metabolites in the human intestine by the PMF-metabolizing bacterium, Blautia sp. MRG-PMF1. Because the newly formed metabolites can have different biological activities, the pathways and regioselectivity of PMF bioconversion were investigated. Using an anaerobic in vitro study, 12 PMFs, 5,7-dimethoxyflavone (5,7-DMF), 5-hydroxy-7-methoxyflavone (5-OH-7-MF), 3,5,7-trimethoxyflavone (3,5,7-TMF), 5-hydroxy3,7-dimethoxyflavone (5-OH-3,7-DMF), 5,7,4'-trimethoxyflavone (5,7,4'-TMF), 5-hydroxy-7,4'-dimethoxyflavone (5-OH-7,4'DMF), 3,5,7,4'-tetramethoxyflavone (3,5,7,4'-TMF), 5-hydroxy-3,7,4'-trimethoxyflavone (5-OH-3,7,4'-TMF), 5,7,3',4'-tetramethoxyflavone (5,7,3',4'-TMF), 3,5,7,3',4'-pentamethoxyflavone (3,5,7,3',4'-PMF), 5-hydroxy-3,7,3',4'-tetramethoxyflavone (5OH-3,7,3',4'-TMF), and 5,3'-dihydroxy-3,7,4'-trimethoxyflavone (5,3'-diOH-3,7,4'-TMF), were converted to chrysin, apigenin, galangin, kaempferol, luteolin, and quercetin after complete demethylation. The time-course monitoring of PMF biotransformation elucidated bioconversion pathways, including the identification of metabolic intermediates. As a robust flavonoid demethylase, regioselectivity of PMF demethylation generally followed the order C-7 > C-4' approximate to C-3' > C-5 > C-3. PMF demethylase in the MRG-PMF1 strain was suggested as a Co-corrinoid methyltransferase system, and this was supported by the experiments utilizing other methyl aryl ether substrates and inhibitors. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Demethylation of Polymethoxyflavones by Human Gut Bacterium, Blautia sp MRG-PMF1 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acsofc7b00408 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.65, no.8, pp 1620 - 1629 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000395493500020 | - |
dc.identifier.scopusid | 2-s2.0-85014184358 | - |
dc.citation.endPage | 1629 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1620 | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 65 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | biotransformation | - |
dc.subject.keywordAuthor | Blautia | - |
dc.subject.keywordAuthor | Co-corrinoid | - |
dc.subject.keywordAuthor | flavonoid | - |
dc.subject.keywordAuthor | human intestinal bacteria | - |
dc.subject.keywordAuthor | polymethoxyflavone | - |
dc.subject.keywordAuthor | regioselectivity | - |
dc.subject.keywordPlus | HUMAN INTESTINAL BACTERIA | - |
dc.subject.keywordPlus | KAEMPFERIA-PARVIFLORA | - |
dc.subject.keywordPlus | ISOFLAVAN-4-OL STEREOISOMERS | - |
dc.subject.keywordPlus | ABSOLUTE-CONFIGURATION | - |
dc.subject.keywordPlus | MICROBIOTA | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | POLYPHENOLS | - |
dc.subject.keywordPlus | (3S)-EQUOL | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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