Characterization of immortalized human corneal endothelial cell line using HPV 16 E6/E7 on lyophilized human amniotic membrane.
DC Field | Value | Language |
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dc.contributor.author | Kim, H.J. | - |
dc.contributor.author | Ryu, Y.H. | - |
dc.contributor.author | Ahn, J.I. | - |
dc.contributor.author | Park, J.K. | - |
dc.contributor.author | Kim, J.C. | - |
dc.date.accessioned | 2021-06-18T13:42:43Z | - |
dc.date.available | 2021-06-18T13:42:43Z | - |
dc.date.issued | 2006-03 | - |
dc.identifier.issn | 1011-8942 | - |
dc.identifier.issn | 2092-9382 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47061 | - |
dc.description.abstract | PURPOSE: To establish the immortalized human corneal endothelial cell line (IHCEn) by transducing human papilloma virus (HPV) 16 E6/E7 oncogenes, and to identify their characteristics when cultivated on a lyophilized human amniotic membrane (LAM). METHODS: Primary human corneal endothelial cells (PHCEn) were infected using a retroviral vector with HPV 16 E6/E7, and transformed cells were clonally selected by G418. Growth properties and characteristics of IHCEn were compared with PHCEn by cell counting and RT-PCR of VDAC3, SLC4A4, CLCN3, FGF-1, Col IV, and Na+/K+ ATPase. IHCEn were cultured on LAM. Messenger RNA expressions of VDAC3, CLCN3, and Na+/K+ ATPase, and protein expressions of Na+/K+ ATPase and Col IV in IHCEn cultivated on LAM were investigated by RT-PCR, immunofluorescence, and immunohistochemical staining, respectively. RESULTS: Successful immortalization was confirmed by stable expression of HPV 16 E6/E7 mRNA by RT-PCR, and IHCEn exhibited typical corneal endothelial morphology. Doubling time of IHCEn was 30.15 +/- 10.96 hrs. Both IHCEn and PHCEn expressed VDAC3, CLCN3, SLC4A4, FGF-1, Col IV, and Na+/K+ ATPase. IHCEn cultivated on LAM showed stronger expression of VDAC3, CLCN4, and Na+/K+ ATPase mRNA than on plastic culture dish. Immunohistochemical staining and immunofluorescence revealed the positive expression of Na+/K+ ATPase and Col IV. CONCLUSIONS: IHCEn were successfully established, and LAM is a good substrate for the culture of human corneal endothelial cells. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한안과학회 | - |
dc.title | Characterization of immortalized human corneal endothelial cell line using HPV 16 E6/E7 on lyophilized human amniotic membrane. | - |
dc.type | Article | - |
dc.identifier.doi | 10.3341/kjo.2006.20.1.47 | - |
dc.identifier.bibliographicCitation | Korean journal of ophthalmology : KJO, v.20, no.1, pp 47 - 54 | - |
dc.identifier.kciid | ART001259569 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-33745625700 | - |
dc.citation.endPage | 54 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 47 | - |
dc.citation.title | Korean journal of ophthalmology : KJO | - |
dc.citation.volume | 20 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Characterization | - |
dc.subject.keywordAuthor | HPV 16 E6/E7 | - |
dc.subject.keywordAuthor | Immortalization | - |
dc.subject.keywordAuthor | Immortalized human corneal endothelial cell lines | - |
dc.subject.keywordAuthor | Lyophilized human amniotic membrane | - |
dc.subject.keywordAuthor | Characterization | - |
dc.subject.keywordAuthor | HPV 16 E6/E7 | - |
dc.subject.keywordAuthor | Immortalization | - |
dc.subject.keywordAuthor | Immortalized human corneal endothelial cell lines | - |
dc.subject.keywordAuthor | Lyophilized human amniotic membrane | - |
dc.subject.keywordPlus | E6 protein, Human papillomavirus type 16 | - |
dc.subject.keywordPlus | messenger RNA | - |
dc.subject.keywordPlus | oncoprotein | - |
dc.subject.keywordPlus | protein tyrosine kinase | - |
dc.subject.keywordPlus | repressor protein | - |
dc.subject.keywordPlus | amnion | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell count | - |
dc.subject.keywordPlus | cell line | - |
dc.subject.keywordPlus | comparative study | - |
dc.subject.keywordPlus | cornea endothelium | - |
dc.subject.keywordPlus | cytology | - |
dc.subject.keywordPlus | drug effect | - |
dc.subject.keywordPlus | freeze drying | - |
dc.subject.keywordPlus | gene expression regulation | - |
dc.subject.keywordPlus | genetic transfection | - |
dc.subject.keywordPlus | genetics | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | immunohistochemistry | - |
dc.subject.keywordPlus | in vitro study | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | reverse transcription polymerase chain reaction | - |
dc.subject.keywordPlus | Amnion | - |
dc.subject.keywordPlus | Cell Count | - |
dc.subject.keywordPlus | Cell Line, Transformed | - |
dc.subject.keywordPlus | Endothelium, Corneal | - |
dc.subject.keywordPlus | Freeze Drying | - |
dc.subject.keywordPlus | Gene Expression Regulation, Viral | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Immunohistochemistry | - |
dc.subject.keywordPlus | Oncogene Proteins, Viral | - |
dc.subject.keywordPlus | Protein-Tyrosine Kinases | - |
dc.subject.keywordPlus | Repressor Proteins | - |
dc.subject.keywordPlus | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.keywordPlus | RNA, Messenger | - |
dc.subject.keywordPlus | Transfection | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | domestic | - |
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