Evaluation of gyrB as chromosomal marker in Bacillus anthracis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, S. | - |
dc.contributor.author | Ryu, C. | - |
dc.contributor.author | Oh, H. | - |
dc.contributor.author | Song, C. | - |
dc.contributor.author | Seong, W.K. | - |
dc.date.accessioned | 2021-06-18T13:43:38Z | - |
dc.date.available | 2021-06-18T13:43:38Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1598-2467 | - |
dc.identifier.issn | 2093-0429 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47131 | - |
dc.description.abstract | Bacillus anthracis is generally accepted as the most potent biological warfare agent because of its highly pathogenic nature and transmission efficiency. Identification of chromosomal markers for the rapid detection of B. anthracis is difficult since significant chromosomal homology exists among B. anthracis, B. cereus and B. thuringiensis. In this study, we tested whether the gyrB sequence could be used as the target for the PCR detection of B. anthracis. The gyrB sequence, composed of 1,923 bp, was identical in 17 Korean B. anthracis isolates. The comparison of gyrB sequence between B. anthracis and B. cereus type strain showed 8.8% difference (105 bp among 1,194 bp), and the gyrB sequence similarities of B. cereus, B. thuringiensis and B. mycoides with B. anthracis were 92.3%, 86.9% and 86.1%, respectively. When polymerase chain reaction was designed and performed based on the gyrB sequence, a specific amplicon (351 bp) could be amplified. These results indicate that gyrB could be useful as a chromosomal marker for the rapid screening of B. anthracis by PCR or differentiation of B. anthracis from other related species by multiplex PCR with other plasmid markers. | - |
dc.format.extent | 10 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.publisher | The Korean Society for Mocrobiology / The Korean Society of Virology | - |
dc.title | Evaluation of gyrB as chromosomal marker in Bacillus anthracis | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | Journal of Bacteriology and Virology, v.34, no.3, pp 191 - 200 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-24044433924 | - |
dc.citation.endPage | 200 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 191 | - |
dc.citation.title | Journal of Bacteriology and Virology | - |
dc.citation.volume | 34 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Bacillus anthracis | - |
dc.subject.keywordAuthor | Chromosomal marker | - |
dc.subject.keywordAuthor | gyrB | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | DNA 16S | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | Bacillus anthracis | - |
dc.subject.keywordPlus | Bacillus cereus | - |
dc.subject.keywordPlus | Bacillus megaterium | - |
dc.subject.keywordPlus | Bacillus subtilis | - |
dc.subject.keywordPlus | Bacillus thuringiensis | - |
dc.subject.keywordPlus | bacterium isolate | - |
dc.subject.keywordPlus | biological warfare | - |
dc.subject.keywordPlus | chromosome marker | - |
dc.subject.keywordPlus | Clostridium botulinum | - |
dc.subject.keywordPlus | Clostridium perfringens | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.subject.keywordPlus | gene sequence | - |
dc.subject.keywordPlus | gyrB gene | - |
dc.subject.keywordPlus | Korea | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | nucleotide sequence | - |
dc.subject.keywordPlus | polymerase chain reaction | - |
dc.subject.keywordPlus | strain difference | - |
dc.subject.keywordPlus | Bacillus anthracis | - |
dc.subject.keywordPlus | Bacillus cereus | - |
dc.subject.keywordPlus | Bacillus megaterium | - |
dc.subject.keywordPlus | Bacillus subtilis | - |
dc.subject.keywordPlus | Bacillus thuringiensis | - |
dc.subject.keywordPlus | Clostridium botulinum | - |
dc.subject.keywordPlus | Clostridium perfringens | - |
dc.subject.keywordPlus | Escherichia coli | - |
dc.description.journalRegisteredClass | scopus | - |
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