Heat shock protein expression in adenosine triphosphate depleted renal epithelial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, D.-J. | - |
dc.contributor.author | Yu, S.-H. | - |
dc.contributor.author | Kang, E.-T. | - |
dc.date.accessioned | 2021-06-18T13:43:41Z | - |
dc.date.available | 2021-06-18T13:43:41Z | - |
dc.date.issued | 2004-09 | - |
dc.identifier.issn | 1226-3303 | - |
dc.identifier.issn | 2005-6648 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47134 | - |
dc.description.abstract | Background: In this study, the putative interactions between apoptosis and heat shock proteins disturbed as a result of ATP depletion were investigated as a hypoxia model. Methods: The direct cellular damages were assessed by the release of LDH from the cytoplasm of the human tubular epithelial cells (HK-2 cells) following ATP depletion. The Bcl-2/Bax mRNA expression ratio, used as an index to assess to what extent apoptosis contributed to tubular cell damage, and the expressions of HSP 90, 72 and 27 in relation to the Bcl-2/Bax ratio in the ischemic model, as parameters of their functional contributions to tubule cell damage, were also studied. Heat preconditioning (HS) was performed at 43°C in a temperature-regulated water bath for 1 h. Results: The release of LDH due to ATP depletion was not significantly increased in HK-2 cells compared to the control, but was slightly increased in heat preconditioned cells compared to non heat preconditioned cells, but the difference was not statistically significant (6.33±0.57 U/L vs. 8.67±2.52 U/L, p>0.05). The Bcl-2/ Bax mRNA expression ratio increased progressively from the control to the heat preconditioned and ATP depleted cells (control; 100%, ATP depletion; 154±6%, heat preconditioning; 212±6%, heat preconditioning and ATP depletion; 421±8%). No contribution of heat preconditioning and ATP depletion was observed on the expressions of HSP90 and HSP27. However, HSP72 expression was prominent by ATP depletion, especially after heat preconditioning. Conclusion: There may be a possibility that the preservation of cytolytic damage and an increase in the Bcl-2/Bax mRNA expression ratio is related to the increase of HSP72 in ATP depletion as a hypoxia model. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Association of Internal Medicine | - |
dc.title | Heat shock protein expression in adenosine triphosphate depleted renal epithelial cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3904/kjim.2004.19.3.149 | - |
dc.identifier.bibliographicCitation | Korean Journal of Internal Medicine, v.19, no.3, pp 149 - 154 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-16544383029 | - |
dc.citation.endPage | 154 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 149 | - |
dc.citation.title | Korean Journal of Internal Medicine | - |
dc.citation.volume | 19 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Bcl-2 | - |
dc.subject.keywordAuthor | Heat shock protein | - |
dc.subject.keywordPlus | adenosine triphosphate | - |
dc.subject.keywordPlus | heat shock protein | - |
dc.subject.keywordPlus | heat shock protein 27 | - |
dc.subject.keywordPlus | heat shock protein 72 | - |
dc.subject.keywordPlus | heat shock protein 90 | - |
dc.subject.keywordPlus | lactate dehydrogenase | - |
dc.subject.keywordPlus | messenger RNA | - |
dc.subject.keywordPlus | protein Bax | - |
dc.subject.keywordPlus | protein bcl 2 | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell hypoxia | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | enzyme release | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | kidney ischemia | - |
dc.subject.keywordPlus | kidney tubule cell | - |
dc.subject.keywordPlus | kidney tubule damage | - |
dc.subject.keywordPlus | kidney tubule epithelium | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | anoxia | - |
dc.subject.keywordPlus | cytology | - |
dc.subject.keywordPlus | epithelium | - |
dc.subject.keywordPlus | kidney tubule | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | Adenosine Triphosphate | - |
dc.subject.keywordPlus | Anoxia | - |
dc.subject.keywordPlus | Epithelium | - |
dc.subject.keywordPlus | Heat-Shock Proteins | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Kidney Tubules | - |
dc.subject.keywordPlus | L-Lactate Dehydrogenase | - |
dc.subject.keywordPlus | RNA, Messenger | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.