Involvement of caspase-3 in apoptosis induced by Viscum album var. coloratum agglutinin in HL-60 cells
DC Field | Value | Language |
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dc.contributor.author | Lyu, SY | - |
dc.contributor.author | Park, WB | - |
dc.contributor.author | Choi, KH | - |
dc.contributor.author | Kim, WH | - |
dc.date.accessioned | 2021-06-18T14:42:05Z | - |
dc.date.available | 2021-06-18T14:42:05Z | - |
dc.date.issued | 2001-03 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.issn | 1347-6947 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47271 | - |
dc.description.abstract | A cytotoxic lectin (Viscum album L. coloratum agglutinin, VCA) from Korean mistletoe was isolated by affinity chromatography on Sepharose 4B immobilized with asialofetuin. In HL-60 cells, addition of VCA resulted in a dose- and time-dependent growth suppression, morphological changes of apoptotic nuclei, and DNA fragmentation characteristics of apoptosis, To investigate how caspase-3 activation during VCA-induced apoptosis induces cleavages of PARP, the expression of PARP and the pattern of caspase-3 activation in HL-60 cells were investigated. The native and processed PARP forms typically seen in apoptotic cells were observed, and a decrease in expression of the 32-kDa form of caspase-3 in a dose-dependent manner was observed. The VCA-induced apoptosis was significantly inhibited by a caspase-3 specific inhibitor, z-DEVD-FMK, and the PARP processing and caspase-3 activation were also inhibited by the inhibitor. A possible involvement of cell cycle arrest in VCA-induced apoptosis was investigated by flow cytometry and the results suggested that the apoptotic effect of VCA is not involved in the induction of cell cycle arrest. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.title | Involvement of caspase-3 in apoptosis induced by Viscum album var. coloratum agglutinin in HL-60 cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1271/bbb.65.534 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.65, no.3, pp 534 - 541 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000167949500008 | - |
dc.identifier.scopusid | 2-s2.0-0035293795 | - |
dc.citation.endPage | 541 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 534 | - |
dc.citation.title | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.citation.volume | 65 | - |
dc.type.docType | Article | - |
dc.publisher.location | 일본 | - |
dc.subject.keywordAuthor | Korean mistletoe | - |
dc.subject.keywordAuthor | lectin | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | caspase-3 | - |
dc.subject.keywordAuthor | poly(ADP-ribose)polymerase | - |
dc.subject.keywordPlus | GALACTOSIDE-SPECIFIC LECTIN | - |
dc.subject.keywordPlus | MISTLETOE EXTRACT | - |
dc.subject.keywordPlus | GLYCOSYLATION PATTERNS | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | A-CHAIN | - |
dc.subject.keywordPlus | B-CHAIN | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | LYMPHOCYTES | - |
dc.subject.keywordPlus | MICE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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