Cell cycle arrest induced by the vitamin D-3 analog EB1089 in NCI-H929 myeloma cells is associated with induction of the cyclin-dependent kinase inhibitor p27
DC Field | Value | Language |
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dc.contributor.author | Park, WH | - |
dc.contributor.author | Seol, JG | - |
dc.contributor.author | Kim, ES | - |
dc.contributor.author | Jung, CW | - |
dc.contributor.author | Lee, CC | - |
dc.contributor.author | Binderup, L | - |
dc.contributor.author | Koeffler, HP | - |
dc.contributor.author | Kim, BK | - |
dc.contributor.author | Lee, YY | - |
dc.date.accessioned | 2021-06-18T14:43:55Z | - |
dc.date.available | 2021-06-18T14:43:55Z | - |
dc.date.issued | 2000-02 | - |
dc.identifier.issn | 0014-4827 | - |
dc.identifier.issn | 1090-2422 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47383 | - |
dc.description.abstract | EB1089, a 1,25-dihydroxyvitamin D-3 analog, has been known to have potent antiproliferative properties in a variety of malignant cells in vitro and in vivo. In the present study, we analyzed the effect of EB1089 on human myeloma cell lines, EB1089 inhibited the proliferation of NCI-H929 cells and RPMI8226 cells in a dose-dependent manner among three myeloma cell lines tested. The antiproliferative effect of EB1089 on myeloma cells was related to the expression level of vitamin D receptor, To investigate the mechanism of the antiproliferative effect of EB1089, cell cycle analysis was attempted in EB1089-sensitive NCI-H929 cells, EB1089 (1 x 10(-8) M) efficiently induced G(1), arrest of the cell cycle. Analysis of G(1) regulatory proteins demonstrated that protein levels of CDK2, CDK4, cyclin D1, and cyclin A were decreased in a time-dependent manner, but not those of CDK6 and cyclin E, by EB1089, In addition, EB1089 (1 x 10(-8) M, 72 h) increased the protein level of the CDKI, p27 and markedly enhanced the binding of p27 with CDK2 compared to EB1089-untreated cells. Furthermore, the activity of CDK2-associated cyclin kinase was decreased, which was accompanied by the reduction of cyclin-D1-, cyclin-E-, and cyclin-A-associated kinase activities, resulting in the hypophosphorylation of Rb protein. These results suggest that EB1089 can inhibit the proliferation of human myeloma cells, especially NCI-H929 cells, via a G(1), block in association with the induction of p27 and the reduction of CDK2 activity, (C) 2000 Academic Press. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC | - |
dc.title | Cell cycle arrest induced by the vitamin D-3 analog EB1089 in NCI-H929 myeloma cells is associated with induction of the cyclin-dependent kinase inhibitor p27 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1006/excr.1999.4735 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL CELL RESEARCH, v.254, no.2, pp 279 - 286 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000085131600010 | - |
dc.identifier.scopusid | 2-s2.0-0009634071 | - |
dc.citation.endPage | 286 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 279 | - |
dc.citation.title | EXPERIMENTAL CELL RESEARCH | - |
dc.citation.volume | 254 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | vitamin D-3 | - |
dc.subject.keywordAuthor | analog | - |
dc.subject.keywordAuthor | cell cycle | - |
dc.subject.keywordAuthor | cyclin-dependent kinase inhibitor | - |
dc.subject.keywordAuthor | p27 | - |
dc.subject.keywordAuthor | myeloma | - |
dc.subject.keywordPlus | BREAST-CANCER CELLS | - |
dc.subject.keywordPlus | LEUKEMIA-CELLS | - |
dc.subject.keywordPlus | PROSTATE-CANCER | - |
dc.subject.keywordPlus | 1,25-DIHYDROXYVITAMIN D-3 | - |
dc.subject.keywordPlus | RECEPTOR EXPRESSION | - |
dc.subject.keywordPlus | GROWTH-INHIBITION | - |
dc.subject.keywordPlus | SURVIVAL-TIME | - |
dc.subject.keywordPlus | A PROMOTER | - |
dc.subject.keywordPlus | LINES | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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