N-Ethylmaleimide modulation of tetrodotoxin-sensitive and tetrodotoxin-resistant sodium channels in rat dorsal root ganglion neurons
DC Field | Value | Language |
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dc.contributor.author | Song, Jin-Ho | - |
dc.contributor.author | Jang, Yoon Young | - |
dc.contributor.author | Shin, Yong Kyoo | - |
dc.contributor.author | Lee, Chung-Soo | - |
dc.contributor.author | Chung, Sungkwon | - |
dc.date.accessioned | 2021-06-18T14:43:56Z | - |
dc.date.available | 2021-06-18T14:43:56Z | - |
dc.date.issued | 2000-02 | - |
dc.identifier.issn | 0006-8993 | - |
dc.identifier.issn | 1872-6240 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47384 | - |
dc.description.abstract | The effects of N-ethylmaleimide (NEM), an alkylating reagent to protein sulfhydryl groups, on tetrodotoxin-sensitive (TTX-S) and tetrodotoxin-resistant (TTX-R) sodium channels in rat, dorsal root ganglion (DRG) neurons were studied using the whole cell configuration of patch-clamp technique. When currents were evoked by step depolarizations to 0 mV from a holding potential of -80 mV NEM decreased the amplitude of TTX-S sodium current, but exerted little or no effect on that of TTX-R sodium current. The inhibitory effect of NEM on TTX-S sodium channel was mainly due to the shift of the steady-stare inactivation curve in the hyperpolarizing direction. NEM did not affect the voltage-dependence of the activation of TTX-S sodium channel. The steady-state inactivation curve for TTX-R sodium channel was shifted by NEM in the hyperpolarizing direction as that for TTX-S sodium channel. NEM caused a change in the voltage-dependence of the activation of TTX-R sodium channel unlike TTX-S sodium channel. After NEM treatment, the amplitudes of TTX-R sodium currents at test voltages below -10 mV were increased, but those at more positive voltages were not affected, This was explained by the shift in the conductance-voltage curve for TTX-R sodium channels in the hyperpolarizing direction after NEM treatment. (C) 2000 Elsevier Science B.V. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | N-Ethylmaleimide modulation of tetrodotoxin-sensitive and tetrodotoxin-resistant sodium channels in rat dorsal root ganglion neurons | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/S0006-8993(99)02405-1 | - |
dc.identifier.bibliographicCitation | BRAIN RESEARCH, v.855, no.2, pp 267 - 273 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000085295700009 | - |
dc.identifier.scopusid | 2-s2.0-0034645942 | - |
dc.citation.endPage | 273 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 267 | - |
dc.citation.title | BRAIN RESEARCH | - |
dc.citation.volume | 855 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | N-ethylmaleimide | - |
dc.subject.keywordAuthor | sulfhydryl | - |
dc.subject.keywordAuthor | alkylation | - |
dc.subject.keywordAuthor | tetrodotoxin-sensitive | - |
dc.subject.keywordAuthor | tetrodotoxin-resistant | - |
dc.subject.keywordAuthor | sodium channel | - |
dc.subject.keywordAuthor | dorsal root ganglion | - |
dc.subject.keywordPlus | CA2+ RELEASE CHANNEL | - |
dc.subject.keywordPlus | SENSORY NEURONS | - |
dc.subject.keywordPlus | VENTRICULAR MYOCYTES | - |
dc.subject.keywordPlus | ION CHANNELS | - |
dc.subject.keywordPlus | NA+ CHANNELS | - |
dc.subject.keywordPlus | K+ CHANNELS | - |
dc.subject.keywordPlus | G-PROTEINS | - |
dc.subject.keywordPlus | CONDUCTANCE | - |
dc.subject.keywordPlus | CURRENTS | - |
dc.subject.keywordPlus | INACTIVATION | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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