Antioxidant and cytoprotective effects of morin against hydrogen peroxide-induced oxidative stress are associated with the induction of Nrf-2-mediated HO-1 expression in V79-4 Chinese hamster lung fibroblasts
DC Field | Value | Language |
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dc.contributor.author | Lee, Moon Hee | - |
dc.contributor.author | Cha, Hee-Jae | - |
dc.contributor.author | Choi, Eun Ok | - |
dc.contributor.author | Han, Min Ho | - |
dc.contributor.author | Kim, Sung Ok | - |
dc.contributor.author | Kim, Gi-Young | - |
dc.contributor.author | Hong, Su Hyun | - |
dc.contributor.author | Park, Cheol | - |
dc.contributor.author | Moon, Sung-Kwon | - |
dc.contributor.author | Jeong, Soon-Jeong | - |
dc.contributor.author | Jeong, Moon-Jin | - |
dc.contributor.author | Kim, Wun-Jae | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.date.available | 2019-03-08T09:36:36Z | - |
dc.date.issued | 2017-03 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.issn | 1791-244X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4742 | - |
dc.description.abstract | Natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. The present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the Moraceae family. Superoxide dismutase (SOD)-like activity and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(center dot+)) radical scavenging activity were determined. We also investigated the cytoprotective effects of morin against hydrogen peroxide (H2O2)-induced DNA damage and apoptosis in V79-4 Chinese hamster lung fibroblasts. Our results demonstrated that morin had strong scavenging effects against ABTS' radicals with enhanced SOD activity, which varied in a dose-dependent manner. Morin was found to reduce H2O2-induced intracellular reactive oxygen species generation and nuclear DNA damage, and it recovered cell viability damaged by H2O2 via inhibition of mitochondrial dysfunction-mediated apoptosis. Notably, the treatment of V79-4 cells with morin markedly enhanced the expression of heme oxygenase-1 (HO-1) but not quinone oxidoreductase-1, which was associated with the increased expression and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the downregulation of Kelch-like ECH-associated protein 1 expression. Based on our findings, we conclude that morin effectively ameliorated oxidative stress-induced DNA damage through intrinsic free radical scavenging activity and activation of the Nrf2/HO-1 pathway. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.title | Antioxidant and cytoprotective effects of morin against hydrogen peroxide-induced oxidative stress are associated with the induction of Nrf-2-mediated HO-1 expression in V79-4 Chinese hamster lung fibroblasts | - |
dc.type | Article | - |
dc.identifier.doi | 10.3892/ijmm.2017.2871 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.39, no.3, pp 672 - 680 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000397173200021 | - |
dc.identifier.scopusid | 2-s2.0-85013040919 | - |
dc.citation.endPage | 680 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 672 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.volume | 39 | - |
dc.type.docType | Article | - |
dc.publisher.location | 그리이스 | - |
dc.subject.keywordAuthor | morin | - |
dc.subject.keywordAuthor | antioxidant | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | Nrf2/HO-1 | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | FREE-RADICALS | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | CARCINOGENS | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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