Meteorin-like protein (METRNL)/IL-41 improves LPS-induced inflammatory responses via AMPK or PPARδ–mediated signaling pathways
DC Field | Value | Language |
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dc.contributor.author | Jung, Tae Woo | - |
dc.contributor.author | Pyun, Do Hyeon | - |
dc.contributor.author | Kim, Tae Jin | - |
dc.contributor.author | Lee, Hyun Jung | - |
dc.contributor.author | Park, Eon Sub | - |
dc.contributor.author | Abd El-Aty, A.M. | - |
dc.contributor.author | Hwang, Eui Jin | - |
dc.contributor.author | Shin, Yong Kyoo | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.date.accessioned | 2021-06-21T07:40:19Z | - |
dc.date.available | 2021-06-21T07:40:19Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 1896-1126 | - |
dc.identifier.issn | 1898-4002 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47495 | - |
dc.description.abstract | Purpose: Meteorin-like protein (METRNL) (also known as IL-41), recently identified as a myokine, is released in response to muscle contraction. It improves the skeletal muscle insulin sensitivity through exerting a beneficial anti-inflammatory effect. However, no independent studies have been published to verify the effects of METRNL on human umbilical vein endothelial cells (HUVECs) and THP-1 human monocytes. Materials and methods: The levels of NFκB and IκB phosphorylation as well as the expression of adhesion molecules were assessed by Western blotting analysis. Cell adhesion assay demonstrated the interactions between HUVEC and THP-1 cells. We used enzyme-linked immunosorbent assay (ELISA) to measure the levels of TNFα and MCP-1 in culture medium. Results: Treatment with METRNL suppressed the secretion of TNFα and MCP-1 as well as NFκB and IκB phosphorylation and inflammatory markers in lipopolysaccharide (LPS)-treated HUVECs and THP-1 cells. Furthermore, treatment with METRNL ameliorated LPS-induced attachment of THP-1 monocytes to HUVECs via inhibition of adhesion molecule expression and apoptosis. Treatment of HUVEC and THP-1 cells with METRNL enhanced AMPK phosphorylation and PPARδ expression in a dose-dependent manner. Small interference (si) RNA-mediated suppression of AMPK or PPARδ restored all these changes. Conclusions: It has therefore been shown that METRNL ameliorates inflammatory responses through AMPK and PPARδ-dependent pathways in LPS-treated HUVEC. In sum, the current study may suggest the suppressive potential of METRNL against endothelial inflammation. © 2021 Medical University of Bialystok | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Medical University of Bialystok | - |
dc.title | Meteorin-like protein (METRNL)/IL-41 improves LPS-induced inflammatory responses via AMPK or PPARδ–mediated signaling pathways | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.advms.2021.01.007 | - |
dc.identifier.bibliographicCitation | Advances in Medical Sciences, v.66, no.1, pp 155 - 161 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000646218100019 | - |
dc.identifier.scopusid | 2-s2.0-85100833917 | - |
dc.citation.endPage | 161 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 155 | - |
dc.citation.title | Advances in Medical Sciences | - |
dc.citation.volume | 66 | - |
dc.type.docType | Article | - |
dc.publisher.location | 폴란드 | - |
dc.subject.keywordAuthor | AMPK | - |
dc.subject.keywordAuthor | HUVEC | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | METRNL | - |
dc.subject.keywordAuthor | PPARδ | - |
dc.subject.keywordAuthor | THP-1 | - |
dc.subject.keywordPlus | caspase 3 | - |
dc.subject.keywordPlus | cytokine | - |
dc.subject.keywordPlus | endothelial leukocyte adhesion molecule 1 | - |
dc.subject.keywordPlus | hydroxymethylglutaryl coenzyme A reductase kinase | - |
dc.subject.keywordPlus | intercellular adhesion molecule 1 | - |
dc.subject.keywordPlus | interleukin 41 | - |
dc.subject.keywordPlus | lipopolysaccharide | - |
dc.subject.keywordPlus | meteorin like protein | - |
dc.subject.keywordPlus | monocyte chemotactic protein 1 | - |
dc.subject.keywordPlus | peroxisome proliferator activated receptor gamma | - |
dc.subject.keywordPlus | small interfering RNA | - |
dc.subject.keywordPlus | tumor necrosis factor | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | vascular cell adhesion molecule 1 | - |
dc.subject.keywordPlus | AMPK signaling | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | antiinflammatory activity | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | cell adhesion assay | - |
dc.subject.keywordPlus | cell viability | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | data analysis software | - |
dc.subject.keywordPlus | enzyme linked immunosorbent assay | - |
dc.subject.keywordPlus | gene knockdown | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | lipopolysaccharide-induced inflammation | - |
dc.subject.keywordPlus | monocyte | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | protein phosphorylation | - |
dc.subject.keywordPlus | THP-1 cell line | - |
dc.subject.keywordPlus | umbilical vein endothelial cell | - |
dc.subject.keywordPlus | Western blotting | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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