Development of p-Coumaric Acid Analysis in Human Plasma and Its Clinical Application to PK/PD Study
DC Field | Value | Language |
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dc.contributor.author | Kim, Hohyun | - |
dc.contributor.author | Choi, Yunkyoung | - |
dc.contributor.author | An, Yongwun | - |
dc.contributor.author | Jung, Young-Rim | - |
dc.contributor.author | Lee, Jin-Yong | - |
dc.contributor.author | Lee, Hong-Jin | - |
dc.contributor.author | Jeong, Jihoon | - |
dc.contributor.author | Kim, Zisoo | - |
dc.contributor.author | Kim, Kyeongsoon | - |
dc.date.accessioned | 2021-06-21T07:40:27Z | - |
dc.date.available | 2021-06-21T07:40:27Z | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 2077-0383 | - |
dc.identifier.issn | 2077-0383 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47498 | - |
dc.description.abstract | It has been recognized that p-Coumaric acid (p-CA) has protective effects as an antioxidant, anti-inflammatory agent. A sensitive and efficient Liquid Chromatography-Mass Spectrometry (LC-MS) method for maximum determination of p-CA in human plasma has been established using Ultra-performance liquid Chromatography-tandem mass Spectrometry (UPLC-MS/MS). This study provides the developed analysis of p-CA extracted from Bambusae Caulis in Taeniam (BC) to examine the improvement of the treatment p-CA, IGF-1 and Osteocalcin level in human children which are important factors on the growth of children's height through Pharmacokinetics/Pharmacodynamics (PK/PD) model. p-CA and internal standard in a plasma sample were detected by the Multiple Reaction Monitoring (MRM) scan mode with positive ion detection. The sample participating in the study was made of 34 subjects (placebo = 18, treatment = 16). The subjects were enrolled to be randomized to the control group and BC group. Randomized subjects took tested treatment twice a day, three capsules with oral administration (258 mg/capsule) each time after a meal. Standard calibration curves (reproducibility) were constructed and the lower limit of quantitation (LLOQ) for p-CA was found to be 0.2 ng/mL on injection of the sample into the UPLC-MS/MS system. Accuracy and precision were evaluated and the intra-accuracy was 99.2-103.8% with precision of 1.0-5.6% and inter-accuracy was 99.6-108.4% and precision of 1.3-6.4% for p-CA. The method has been successfully applied to PK/PD studies of p-CA in human plasma. The p-CA, BC in Taeniam extract increased the level of IGF-1 and Osteocalcin, and changed the height from baseline, which suggested that the p-CA could play an important role in longitudinal bone growth. Therefore, the p-CA extracted from BC in Taeniam might be a good alternative medicine to growth hormone (GH) therapy. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Development of p-Coumaric Acid Analysis in Human Plasma and Its Clinical Application to PK/PD Study | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/jcm10010108 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL MEDICINE, v.10, no.1, pp 1 - 11 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000606665900001 | - |
dc.identifier.scopusid | 2-s2.0-85111265541 | - |
dc.citation.endPage | 11 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | JOURNAL OF CLINICAL MEDICINE | - |
dc.citation.volume | 10 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | p-Coumaric acid | - |
dc.subject.keywordAuthor | Bambusae Caulis in Taeniam | - |
dc.subject.keywordAuthor | IGF-1 | - |
dc.subject.keywordAuthor | Osteocalcin | - |
dc.subject.keywordAuthor | growth hormone therapy | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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