IL-17C expression and its correlation with pediatric adenoids: a preliminary study
DC Field | Value | Language |
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dc.contributor.author | Min, Hyun Jin | - |
dc.contributor.author | Kim, Kyung Soo | - |
dc.date.accessioned | 2021-07-22T05:01:07Z | - |
dc.date.available | 2021-07-22T05:01:07Z | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 1449-1907 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47731 | - |
dc.description.abstract | Objective: Interleukin-17 (IL-17) C is a cytokine expressed by epithelial cells in response to bacterial stimulation. In contrast to other members of the IL-17 family of cytokines, IL-17C is upregulated early during infection, maintains integrity of the epithelial layer barrier, and mediates the innate immune response. We investigated the expression profile of IL-17C in pediatric adenoids. Methods: Pediatric adenoid tissues and lavage fluids were collected from a total of 38 subjects. The Limulus amebocyte lysate test and real-time PCR using Staphylococcus aureus primers were performed to evaluate bacterial contents in adenoids. Expression of IL-17RE in adenoids was analyzed using real-time polymerase chain reaction and western blot. The expression of IL-17C was evaluated by western blot and immunohistochemistry and compared between allergic rhinitis (AR) and control subjects. The levels of Hsp27, Hsp70, and IL-17C in adenoid lavage fluids were evaluated by enzyme-linked immunosorbent assay, and the correlation between these molecules was statistically analyzed. Results: The pediatric adenoids were found to be exposed to bacteria and had a normal flora comprising both gram-negative and -positive bacteria. IL-17RE, an IL-17C specific receptor, was highly expressed in the epithelium of adenoids. IL-17C was expressed in all evaluated adenoid tissue samples, irrespective of the allergic status of the patient. IL-17C secretion was detected in half of the adenoid lavage fluid samples and was associated with Hsp70 level. Conclusion: Our findings indicate the possible role of pediatric adenoids in innate immunity modulation via an innate immunity-associated cytokine. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | IVYSPRING INT PUBL | - |
dc.title | IL-17C expression and its correlation with pediatric adenoids: a preliminary study | - |
dc.type | Article | - |
dc.identifier.doi | 10.7150/ijms.49244 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, v.17, no.17, pp 2603 - 2610 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000580541400001 | - |
dc.identifier.scopusid | 2-s2.0-85094623235 | - |
dc.citation.endPage | 2610 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 2603 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | - |
dc.citation.volume | 17 | - |
dc.type.docType | Article | - |
dc.publisher.location | 호주 | - |
dc.subject.keywordAuthor | adenoids | - |
dc.subject.keywordAuthor | IL-17C | - |
dc.subject.keywordAuthor | IL-17RE | - |
dc.subject.keywordAuthor | Hsp70 | - |
dc.subject.keywordAuthor | innate immunity | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | CHRONIC RHINOSINUSITIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | SEVERITY | - |
dc.subject.keywordPlus | PROMOTES | - |
dc.subject.keywordPlus | CHILDREN | - |
dc.subject.keywordPlus | FAMILY | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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