Mitogen-Activated Protein Kinase CaDIMK1 Functions as a Positive Regulator of Drought Stress Response and Abscisic Acid Signaling in Capsicum annuumopen access
- Authors
- Kim, Minchae; Jeong, Soongon; Lim, Chae Woo; Lee, Sung Chul
- Issue Date
- 29-Apr-2021
- Publisher
- FRONTIERS MEDIA SA
- Keywords
- ABA; drought; kinase; phosphorylation; stomata
- Citation
- FRONTIERS IN PLANT SCIENCE, v.12
- Journal Title
- FRONTIERS IN PLANT SCIENCE
- Volume
- 12
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47811
- DOI
- 10.3389/fpls.2021.646707
- ISSN
- 1664-462X
- Abstract
- Protein phosphorylation by kinase is an important mechanism for adapting to drought stress conditions. Here, we isolated the CaDIMK1 (Capsicum annuum drought-induced MAP kinase 1) from dehydrated pepper leaf tissue and functionally characterized it. Subcellular localization analysis revealed that the CaDIMK1 protein was localized in the cytoplasm and nucleus. CaDIMK1-silenced pepper plants exhibited drought-susceptible phenotypes that were characterized by increased transpiration rates, low leaf temperatures, and decreased stomatal closure. In contrast, CaDIMK1-overexpressing (OX) transgenic Arabidopsis plants were hypersensitive to abscisic acid (ABA) from germination to adult growth stages. Furthermore, the CaDIMK1-OX plants were tolerant to drought stress. The transcript levels of several stress-related genes were high in CaDIMK1-OX plants than in wild-type plants. Taken together, our data demonstrate that CaDIMK1 acts as a positive modulator of drought tolerance and ABA signal transduction in pepper plants.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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