Plant callus-derived shikimic acid regenerates human skin through converting human dermal fibroblasts into multipotent skin-derived precursor cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Y.-W. | - |
dc.contributor.author | Lee, S.-H. | - |
dc.contributor.author | Kim, A.-R. | - |
dc.contributor.author | Kim, B.J. | - |
dc.contributor.author | Park, W.-S. | - |
dc.contributor.author | Hur, J. | - |
dc.contributor.author | Jang, H. | - |
dc.contributor.author | Yang, H.-M. | - |
dc.contributor.author | Cho, H.-J. | - |
dc.contributor.author | Kim, H.-S. | - |
dc.date.accessioned | 2021-07-29T05:40:07Z | - |
dc.date.available | 2021-07-29T05:40:07Z | - |
dc.date.issued | 2021-06-11 | - |
dc.identifier.issn | 1757-6512 | - |
dc.identifier.issn | 1757-6512 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/47998 | - |
dc.description.abstract | Background: The human skin-derived precursors (SKPs) are a good cell source for regeneration. However, the isolation of SKP from human skin is limited. To overcome this drawback, we hypothesized that the component of plant stem cells could convert human fibroblasts to SKPs. Methods: Human dermal fibroblasts were treated with shikimic acid, a major component of Sequoiadendron giganteum callus extract. The characteristics of these reprogrammed cells were analyzed by qPCR, western blot, colony-forming assay, and immunofluorescence staining. Artificial human skin was used for CO2 laser-induced wound experiments. Human tissues were analyzed by immunohistochemistry. Results: The reprogrammed cells expressed nestin (a neural precursor-specific protein), fibronectin, and vimentin and could differentiate into the ectodermal and mesodermal lineage. Nestin expression was induced by shikimic acid through the mannose receptor and subsequent MYD88 activation, leading to P38 phosphorylation and then CREB binding to the nestin gene promoter. Finally, we confirmed that shikimic acid facilitated the healing of cut injury and enhanced dermal reconstruction in a human artificial skin model. Moreover, in a clinical study with healthy volunteers, plant callus extracts increased the expression of stem cell markers in the basal layer of the epidermis and collagen deposit in the dermis. Conclusions: These results indicate that shikimic acid is an effective agent for tissue regeneration. © 2021, The Author(s). | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BioMed Central Ltd | - |
dc.title | Plant callus-derived shikimic acid regenerates human skin through converting human dermal fibroblasts into multipotent skin-derived precursor cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s13287-021-02409-3 | - |
dc.identifier.bibliographicCitation | Stem Cell Research and Therapy, v.12, no.1 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000663789300001 | - |
dc.identifier.scopusid | 2-s2.0-85107604126 | - |
dc.citation.number | 1 | - |
dc.citation.title | Stem Cell Research and Therapy | - |
dc.citation.volume | 12 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Cell reprogramming | - |
dc.subject.keywordAuthor | Neural precursor | - |
dc.subject.keywordAuthor | Shikimic acid | - |
dc.subject.keywordAuthor | Single chemical-derived trans-differentiation | - |
dc.subject.keywordAuthor | Skin-derived precursor | - |
dc.subject.keywordPlus | collagen | - |
dc.subject.keywordPlus | cyclic AMP responsive element binding protein | - |
dc.subject.keywordPlus | fibronectin | - |
dc.subject.keywordPlus | mannose receptor | - |
dc.subject.keywordPlus | myeloid differentiation factor 88 | - |
dc.subject.keywordPlus | nestin | - |
dc.subject.keywordPlus | shikimic acid | - |
dc.subject.keywordPlus | synaptophysin | - |
dc.subject.keywordPlus | vimentin | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | callus (plant) | - |
dc.subject.keywordPlus | cell differentiation | - |
dc.subject.keywordPlus | cell lineage | - |
dc.subject.keywordPlus | cell regeneration | - |
dc.subject.keywordPlus | clinical effectiveness | - |
dc.subject.keywordPlus | colony forming unit | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | drug effect | - |
dc.subject.keywordPlus | drug efficacy | - |
dc.subject.keywordPlus | drug mechanism | - |
dc.subject.keywordPlus | drug response | - |
dc.subject.keywordPlus | ectoderm | - |
dc.subject.keywordPlus | epidermis | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | immunofluorescence assay | - |
dc.subject.keywordPlus | immunohistochemistry | - |
dc.subject.keywordPlus | mesoderm | - |
dc.subject.keywordPlus | multipotent stem cell | - |
dc.subject.keywordPlus | polymerase chain reaction | - |
dc.subject.keywordPlus | promoter region | - |
dc.subject.keywordPlus | protein binding | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | protein phosphorylation | - |
dc.subject.keywordPlus | Sequoiadendron | - |
dc.subject.keywordPlus | Sequoiadendron giganteum | - |
dc.subject.keywordPlus | skin cell | - |
dc.subject.keywordPlus | skin fibroblast | - |
dc.subject.keywordPlus | stem cell | - |
dc.subject.keywordPlus | Western blotting | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.