6-azauridine induces autophagy-mediated cell death via a p53-and AMPK-dependent pathway
DC Field | Value | Language |
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dc.contributor.author | Cha, Yeo-Eun | - |
dc.contributor.author | Park, Rackhyun | - |
dc.contributor.author | Jang, Minsu | - |
dc.contributor.author | Park, Yea-In | - |
dc.contributor.author | Yamamoto, Ayane | - |
dc.contributor.author | Oh, Won Keun | - |
dc.contributor.author | Lee, Eun-Ju | - |
dc.contributor.author | Park, Junsoo | - |
dc.date.accessioned | 2021-08-13T06:40:15Z | - |
dc.date.available | 2021-08-13T06:40:15Z | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/48371 | - |
dc.description.abstract | 6-Azauridine (6-AZA), a pyrimidine nucleoside analogue, is known to exhibit both anti-tumor and antiviral activities. Although 6-AZA was discovered more than 60 years ago, the cellular effects of this compound are yet to be elucidated. Here, we report that 6-AZA regulates autophagy-mediated cell death in various human cancer cells, where 6-AZA treatment activates autophagic flux through the activation of lysosomal function. Furthermore, 6-AZA exhibited cytotoxicity in all cancer cells studied, although the mechanisms of action were diverse. In H460 cells, 6-AZA treatment induced apoptosis, and the extent of the latter could be reduced by treatment with chloroquine (CQ), a lysosomal inhibitor. However, 6-AZA treatment resulted in cell cycle arrest in H1299 cells, which could not be reversed by CQ. The cytotoxicity associated with 6-AZA treatment could be linearly correlated to the degree of autophagy-mediated cell death. In addition, we demonstrated that the cytotoxic effect of 6-AZA was dependent on AMPK and p53. These results collectively in-dicate that autophagy-mediated cell death triggered by 6-AZA contributes to its antitumor effect. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | 6-azauridine induces autophagy-mediated cell death via a p53-and AMPK-dependent pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms22062947 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, v.22, no.6 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000645754500001 | - |
dc.identifier.scopusid | 2-s2.0-85102363994 | - |
dc.citation.number | 6 | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.volume | 22 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | 6-azauridine | - |
dc.subject.keywordAuthor | Autophagic flux | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | Autophagy-mediated cell death | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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