Surface-Modification of RIPL Peptide-Conjugated Liposomes to Achieve Steric Stabilization and pH Sensitivity
DC Field | Value | Language |
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dc.contributor.author | Kwon, Yie Hyuk | - |
dc.contributor.author | Shin, Taek Hwan | - |
dc.contributor.author | Jang, Moon Ho | - |
dc.contributor.author | Yoon, Ho Yub | - |
dc.contributor.author | Kang, Min Hyung | - |
dc.contributor.author | Kang, Myung Joo | - |
dc.contributor.author | Choi, Young Wook | - |
dc.date.available | 2019-03-08T09:37:44Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.issn | 1533-4899 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4850 | - |
dc.description.abstract | We have previously demonstrated that RIPL peptide-conjugated liposomes (RIPL-L) exhibited high hepsin (HPN) selectivity and enhanced intracellular drug delivery. In this study, surface modification of RIPL-L was performed to reduce plasma protein adsorption and off-target effects. For steric stabilization, distearoyl phosphatidylethanolamine (DSPE)-polyethylene glycol (PEG)(2000) was used (5% molar ratio to total lipid) to prepare PEG-RIPL-L. Further, pH-sensitive oligopeptides [(HD)(4) or (HE)(4)] were coupled to shield the RIPL polyarginine moiety, yielding (HD)(4)/PEG-RIPL-L and (HE)(4)/PEG-RIPL-L. All liposomal vesicles had a narrow and homogenous size distribution of approximately 140-150 nm, with zeta potentials varying from - 15 to 36 mV. Increased plasma stability was observed upon quantifying the protein adsorbed onto liposomes by using a micro bicinchoninic acid assay. The (HD)(4) - and (HE)(4)-coupling capacity of PEG-RIPL-L was investigated by measuring the amount of oligopeptide involved in transient ionic complexation (TIC-oligopep) and zeta potential changes. As the molar ratio of (HD)(4) and (HE)(4) increased, TIC-oligopep increased and zeta potential decreased. (HE)(4)/PEG-RIPL-L were pH- sensitive, producing 1.6-fold greater cellular uptake of FITC-dextran by LNCaP cells at pH 6.8 than at pH 7.4. This result suggested that (HE)(4)/PEG-RIPL-L might provide a sterically stabilized, pH-sensitive drug carrier for HPN-specific cancer targeting. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.title | Surface-Modification of RIPL Peptide-Conjugated Liposomes to Achieve Steric Stabilization and pH Sensitivity | - |
dc.type | Article | - |
dc.identifier.doi | 10.1166/jnn.2017.12670 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.17, no.2, pp 1008 - 1017 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000397118700020 | - |
dc.identifier.scopusid | 2-s2.0-85010032139 | - |
dc.citation.endPage | 1017 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1008 | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 17 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Liposome | - |
dc.subject.keywordAuthor | RIPL Peptide | - |
dc.subject.keywordAuthor | Oligopeptide | - |
dc.subject.keywordAuthor | pH Sensitivity | - |
dc.subject.keywordAuthor | Targeting | - |
dc.subject.keywordAuthor | Surface Modification | - |
dc.subject.keywordAuthor | Cell Uptake | - |
dc.subject.keywordAuthor | Hepsin | - |
dc.subject.keywordAuthor | Nanocarrier | - |
dc.subject.keywordPlus | DRUG-DELIVERY SYSTEM | - |
dc.subject.keywordPlus | CLINICAL-APPLICATIONS | - |
dc.subject.keywordPlus | MEDIATED DELIVERY | - |
dc.subject.keywordPlus | CANCER-THERAPY | - |
dc.subject.keywordPlus | PEG CHAIN | - |
dc.subject.keywordPlus | NANOARCHITECTONICS | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | PEGYLATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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