Characterization of Prototype Foamy Virus Infectivity in Transportin 3 Knockdown Human 293t Cell Line
DC Field | Value | Language |
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dc.contributor.author | Bin Hamid, Faysal | - |
dc.contributor.author | Kim, Jinsun | - |
dc.contributor.author | Shin, Cha-Gyun | - |
dc.date.available | 2019-03-08T09:37:45Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4852 | - |
dc.description.abstract | The foamy viruses are currently considered essential for development as vectors for gene delivery. Previous studies demonstrated that prototype foamy virus (PFV) can infect and replicate prevalently in a variety of cell types for its exclusive replication strategy. However, the virus-host interaction, especially PFV-transportin3 (TNPO3), is still poorly understood. In our investigation of the role of TNPO3 in PFV infection, we found lower virus production in TNPO3 knockdown (KD) cells compared with wild-type 293T cells. PCR analysis revealed that viral DNAs were mostly altered to circular forms: both 1-long terminal repeat (1-LTR) and 2-LTR in TNPO3 KD cells. We therefore suggest that TNPO3 is required for successful PFV replication, at least at/after the nuclear entry step of viral DNA. These findings highlight the obscure mysteries of PFV-host interaction and the requirement of TNPO3 for productive infection of PFV in 293T cells. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Characterization of Prototype Foamy Virus Infectivity in Transportin 3 Knockdown Human 293t Cell Line | - |
dc.type | Article | - |
dc.identifier.doi | 10.4014/jmb.1606.06011 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.27, no.2, pp 380 - 387 | - |
dc.identifier.kciid | ART002201604 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000397168300021 | - |
dc.identifier.scopusid | 2-s2.0-85014351247 | - |
dc.citation.endPage | 387 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 380 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 27 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Transportin-3 | - |
dc.subject.keywordAuthor | prototype foamy virus | - |
dc.subject.keywordAuthor | 1-long terminal repeat | - |
dc.subject.keywordAuthor | 293 T cell | - |
dc.subject.keywordPlus | NUCLEAR-LOCALIZATION | - |
dc.subject.keywordPlus | HIV-1 REPLICATION | - |
dc.subject.keywordPlus | IMPORTIN-BETA | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | DOMAINS | - |
dc.subject.keywordPlus | TNPO3 | - |
dc.subject.keywordPlus | GAG | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | INTEGRASE | - |
dc.subject.keywordPlus | VECTORS | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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