Mismatch intolerance of 5′-truncated sgrnas in crispr/cas9 enables efficient microbial single-base genome editing
DC Field | Value | Language |
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dc.contributor.author | Lee, Ho Joung | - |
dc.contributor.author | Kim, Hyun Ju | - |
dc.contributor.author | Lee, Sang Jun | - |
dc.date.accessioned | 2021-08-27T06:40:18Z | - |
dc.date.available | 2021-08-27T06:40:18Z | - |
dc.date.issued | 2021-06 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/48929 | - |
dc.description.abstract | The CRISPR/Cas9 system has recently emerged as a useful gene-specific editing tool. However, this approach occasionally results in the digestion of both the DNA target and similar DNA sequences due to mismatch tolerance, which remains a significant drawback of current genome editing technologies. However, our study determined that even single-base mismatches between the target DNA and 5′-truncated sgRNAs inhibited target recognition. These results suggest that a 5′-truncated sgRNA/Cas9 complex could be used to negatively select single-base-edited targets in microbial genomes. Moreover, we demonstrated that the 5′-truncated sgRNA method can be used for simple and effective single-base editing, as it enables the modification of individual bases in the DNA target, near and far from the 5′ end of truncated sgRNAs. Further, 5′-truncated sgRNAs also allowed for efficient single-base editing when using an engineered Cas9 nuclease with an expanded protospacer adjacent motif (PAM; 5′-NG), which may enable whole-genome single-base editing. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Mismatch intolerance of 5′-truncated sgrnas in crispr/cas9 enables efficient microbial single-base genome editing | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms22126457 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, v.22, no.12 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000666267300001 | - |
dc.identifier.scopusid | 2-s2.0-85107840667 | - |
dc.citation.number | 12 | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.volume | 22 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | 5′-truncated sgRNA | - |
dc.subject.keywordAuthor | Cas9 | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | Microbial genome editing | - |
dc.subject.keywordAuthor | Single base | - |
dc.subject.keywordPlus | OFF-TARGET | - |
dc.subject.keywordPlus | GUIDE RNA | - |
dc.subject.keywordPlus | CAS9 | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | MUTAGENESIS | - |
dc.subject.keywordPlus | NUCLEASE | - |
dc.subject.keywordPlus | PRECISE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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