Structure-activity relationships of omega-Agatoxin IVA in lipid membranes
DC Field | Value | Language |
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dc.contributor.author | Ryu, Jae Ha | - |
dc.contributor.author | Jung, Hoi Jong | - |
dc.contributor.author | Konishi, Shiro | - |
dc.contributor.author | Kim, Ha Hyung | - |
dc.contributor.author | Park, Zee-Yong | - |
dc.contributor.author | Kim, Jae Il | - |
dc.date.available | 2019-03-08T09:38:35Z | - |
dc.date.issued | 2017-01 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4920 | - |
dc.description.abstract | To analyze structural features of omega-Aga IVA, a gating modifier toxin from spider venom, we here investigated the NMR solution structure of omega-Aga IVA within DPC micelles. Under those conditions, the Cys-rich central region of omega-Aga IVA still retains the inhibitor Cys knot motif with three short antiparallel beta-strands seen in water. However, N-15 HSQC spectra of omega-Aga IVA within micelles revealed that there are radical changes to the toxin's C-terminal tail and several loops upon binding to micelles. The C-terminal tail of omega-Aga IVA appears to assume a beta-turn like conformation within micelles, though it is disordered in water. Whole-cell patch clamp studies with several omega-Aga IVA analogs indicate that both the hydrophobic C-terminal tail and an Arg patch in the core region of omega-Aga IVA are critical for Cav2.1 blockade. These results suggest that the membrane environment stabilizes the structure of the toxin, enabling it to act in a manner similar to other gating modifier toxins, though its mode of interaction with the membrane and the channel is unique. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Structure-activity relationships of omega-Agatoxin IVA in lipid membranes | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2016.11.025 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.482, no.1, pp 170 - 175 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000392461600027 | - |
dc.identifier.scopusid | 2-s2.0-85006973083 | - |
dc.citation.endPage | 175 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 170 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 482 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | omega-Agatoxin IVA | - |
dc.subject.keywordAuthor | Gating modifier toxin | - |
dc.subject.keywordAuthor | Cav2.1 | - |
dc.subject.keywordPlus | DEPENDENT K+ CHANNEL | - |
dc.subject.keywordPlus | CALCIUM-CHANNELS | - |
dc.subject.keywordPlus | VOLTAGE | - |
dc.subject.keywordPlus | ANTAGONIST | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | MODIFIER | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | TOXIN | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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