Gold-copper nanoshell dot-blot immunoassay for naked-eye sensitive detection of tuberculosis specific CFP-10 antigen
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Phan, Le Minh Tu | - |
dc.contributor.author | Rafique, Rafia | - |
dc.contributor.author | Baek, Seung Hoon | - |
dc.contributor.author | Nguyen, Thang Phan | - |
dc.contributor.author | Park, Kyoung Yeol | - |
dc.contributor.author | Kim, Eun Bee | - |
dc.contributor.author | Kim, Jong Gil | - |
dc.contributor.author | Park, Jong Pil | - |
dc.contributor.author | Kailasa, Suresh Kumar | - |
dc.contributor.author | Kim, Hwa-Jung | - |
dc.contributor.author | Chung, Chaeuk | - |
dc.contributor.author | Shim, Tae Sun | - |
dc.contributor.author | Park, Tae Jung | - |
dc.date.available | 2019-01-22T11:35:56Z | - |
dc.date.issued | 2018-12 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/493 | - |
dc.description.abstract | Herein, a straightforward and highly specific dot-blot immunoassay was successfully developed for the detection of Mycobacterium tuberculosis antigen (10 kDa culture filtrate protein, CFP-10) via the formation of copper nanoshell on the gold nanoparticles (AuNPs) surface. The principle of dot-blot immunoassay was based on the reduction of Cu2+ ion on the GBP-CFP1OG2-AuNPs conjugates, which has gold binding and antigen binding affinities, simultaneously, favouring to appear red dot that can be observed with naked-eye. The dot intensity is proportional to the concentration of tuberculosis antigen CFP-10, which offers a detection limit of 7.6 pg/mL. The analytical performance of GBP-CFPIOG2-AuNPs-copper nanoshell dot-blot was superior than that of conventional silver nanoshell. This method was successfully applied to identify the CFP-10 antigen in the clinical urine sample with high sensitivity, specificity, and minimized sample preparation steps. This method exhibits great application potential in the field of nanomedical science for highly reliable point-of-care detection of CFP-10 antigen in real samples to early diagnosis of tuberculosis. | - |
dc.format.extent | 7 | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Gold-copper nanoshell dot-blot immunoassay for naked-eye sensitive detection of tuberculosis specific CFP-10 antigen | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2018.08.068 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.121, pp 111 - 117 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000447819700015 | - |
dc.identifier.scopusid | 2-s2.0-85053038502 | - |
dc.citation.endPage | 117 | - |
dc.citation.startPage | 111 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 121 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | CFP-10 | - |
dc.subject.keywordAuthor | Tuberculosis | - |
dc.subject.keywordAuthor | Gold nanoparticle | - |
dc.subject.keywordAuthor | Copper | - |
dc.subject.keywordAuthor | Signal enhancement | - |
dc.subject.keywordAuthor | Immunoassay | - |
dc.subject.keywordPlus | POINT-OF-CARE | - |
dc.subject.keywordPlus | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject.keywordPlus | MAGNETOPHORETIC IMMUNOASSAY | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | SILVER | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | POLYETHYLENEIMINE | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.