The synaptonemal complex central region modulates crossover pathways and feedback control of meiotic double-strand break formation
DC Field | Value | Language |
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dc.contributor.author | Lee, Min-Su | - |
dc.contributor.author | Higashide, Mika T. | - |
dc.contributor.author | Choi, Hyungseok | - |
dc.contributor.author | Li, Ke | - |
dc.contributor.author | Hong, Soogil | - |
dc.contributor.author | Lee, Kangseok | - |
dc.contributor.author | Shinohara, Akira | - |
dc.contributor.author | Shinohara, Miki | - |
dc.contributor.author | Kim, Keun P. | - |
dc.date.accessioned | 2021-09-17T04:40:31Z | - |
dc.date.available | 2021-09-17T04:40:31Z | - |
dc.date.issued | 2021-07 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.issn | 1362-4962 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49423 | - |
dc.description.abstract | The synaptonemal complex (SC) is a proteinaceous structure that mediates homolog engagement and genetic recombination during meiosis. In budding yeast, Zip-Mer-Msh (ZMM) proteins promote crossover (CO) formation and initiate SC formation. During SC elongation, the SUMOylated SC component Ecm11 and the Ecm11-interacting protein Gmc2 facilitate the polymerization of Zip1, an SC central region component. Through physical recombination, cytological, and genetic analyses, we found that ecm11 and gmc2 mutants exhibit chromosome-specific defects in meiotic recombination. CO frequencies on a short chromosome (chromosome III) were reduced, whereas CO and non-crossover frequencies on a long chromosome (chromosome VII) were elevated. Further, in ecm11 and gmc2 mutants, more double-strand breaks (DSBs) were formed on a long chromosome during late prophase I, implying that the Ecm11-Gmc2 (EG) complex is involved in the homeostatic regulation of DSB formation. The EG complex may participate in joint molecule (JM) processing and/or double-Holliday junction resolution for ZMM-dependent CO-designated recombination. Absence of the EG complex ameliorated the JM-processing defect in zmm mutants, suggesting a role for the EG complex in suppressing ZMM-independent recombination. Our results suggest that the SC central region functions as a compartment for sequestering recombination-associated proteins to regulate meiosis specificity during recombination. © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. | - |
dc.format.extent | 17 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NLM (Medline) | - |
dc.title | The synaptonemal complex central region modulates crossover pathways and feedback control of meiotic double-strand break formation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/nar/gkab566 | - |
dc.identifier.bibliographicCitation | Nucleic acids research, v.49, no.13, pp 7537 - 7553 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000685211300029 | - |
dc.identifier.scopusid | 2-s2.0-85112127020 | - |
dc.citation.endPage | 7553 | - |
dc.citation.number | 13 | - |
dc.citation.startPage | 7537 | - |
dc.citation.title | Nucleic acids research | - |
dc.citation.volume | 49 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | CROSSING-OVER | - |
dc.subject.keywordPlus | ZMM PROTEINS | - |
dc.subject.keywordPlus | RECOMBINATION | - |
dc.subject.keywordPlus | MEIOSIS | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | SGS1 | - |
dc.subject.keywordPlus | INITIATION | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | HELICASE | - |
dc.subject.keywordPlus | INTERMEDIATE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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