Cloning and expression in Pichia pastoris of a new cytochrome P450 gene from a dandruff-causing Malassezia globosa
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, E.C. | - |
dc.contributor.author | Ohk, S.O. | - |
dc.contributor.author | Suh, B.Y. | - |
dc.contributor.author | Park, N. | - |
dc.contributor.author | Kim, B.J. | - |
dc.contributor.author | Kim, D. | - |
dc.contributor.author | Chun, Y.-J. | - |
dc.date.accessioned | 2021-09-17T04:40:57Z | - |
dc.date.available | 2021-09-17T04:40:57Z | - |
dc.date.issued | 2010-03 | - |
dc.identifier.issn | 1976-8257 | - |
dc.identifier.issn | 2234-2753 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49448 | - |
dc.description.abstract | The Malassezia fungi are responsible for various human skin disorders including dandruff and seborrheic dermatitis. Of the Malassezia fungi, Malassezia globosa (M. globosa) is one of the most common in human scalp. The completed genome sequence of M. globosa contains four putative cytochrome P450 genes. To determine the roles of Malassezia P450 enzymes in the biosynthesis of ergosterol, we isolated MGL3996 gene from M. globosa chromosomal DNA by PCR. The MGL3996 gene encodes an enzyme of 616 amino acids, which shows strong similarity with known CYP52s of other species. MGL3996 gene was cloned and expressed in Pichia pastoris (P. pastoris) heterologous yeast expression system. Using the yeast microsomes expressing MGL3996 protein, a typical P450 CO-difference spectrum was shown with absorption maximum at 448 nm. SDS-PAGE analysis revealed a protein band of apparent molecular weight 69 kDa and Western blot with anti-histidine tag antibody showed that MGL3996 was successfully expressed in P. pastoris. Cloning and expression of a new P450 gene is an important step to study the P450 monooxygenase system of M. globosa and to understand the role of P450 enzymes in pathophysiology of dandruff. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국독성학회 | - |
dc.title | Cloning and expression in Pichia pastoris of a new cytochrome P450 gene from a dandruff-causing Malassezia globosa | - |
dc.title.alternative | Cloning and Expression in Pichia pastoris of a New Cytochrome P450 Gene from a Dandruff-causing Malassezia globosa | - |
dc.type | Article | - |
dc.identifier.doi | 10.5487/TR.2010.26.1.047 | - |
dc.identifier.bibliographicCitation | Toxicological Research, v.26, no.1, pp 47 - 52 | - |
dc.identifier.kciid | ART001425089 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84863116552 | - |
dc.citation.endPage | 52 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 47 | - |
dc.citation.title | Toxicological Research | - |
dc.citation.volume | 26 | - |
dc.type.docType | Short Survey | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | CYP52 | - |
dc.subject.keywordAuthor | Dandruff | - |
dc.subject.keywordAuthor | Malassezia fungi | - |
dc.subject.keywordAuthor | P450 | - |
dc.subject.keywordAuthor | Pichia pastoris | - |
dc.subject.keywordPlus | Fungi | - |
dc.subject.keywordPlus | Malassezia | - |
dc.subject.keywordPlus | Malassezia globosa | - |
dc.subject.keywordPlus | Pichia pastoris | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.