Establishment of three-dimensional bioprinted bladder cancer-on-a-chip with a microfluidic system using bacillus calmette–guérin
DC Field | Value | Language |
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dc.contributor.author | Kim, Jung Hoon | - |
dc.contributor.author | Lee, Seungjin | - |
dc.contributor.author | Kang, Su Jeong | - |
dc.contributor.author | Choi, Young Wook | - |
dc.contributor.author | Choi, Se Young | - |
dc.contributor.author | Park, Joong Yull | - |
dc.contributor.author | Chang, In Ho | - |
dc.date.accessioned | 2021-09-17T06:40:18Z | - |
dc.date.available | 2021-09-17T06:40:18Z | - |
dc.date.issued | 2021-08 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49480 | - |
dc.description.abstract | Immunotherapy of bladder cancer is known to have favorable effects, although it is difficult to determine which patients will show a good response because of the different tumor microenvi-ronments (TME). Here, we developed a bladder cancer-on-a-chip (BCOC) to mimic the TME using three-dimensional (3D) bioprinting and microfluidic technology. We fabricated a T24 and a 5637-cell line-based BCOC that also incorporated MRC-5, HUVEC, and THP-1 cells. We evaluated the effects of TME and assessed the immunologic reactions in response to different concentrations of Bacillus Calmette–Guérin (BCG) via live/dead assay and THP-1 monocytic migration, and concentrations of growth factors and cytokines. The results show that cell viability was maintained at 15% filling density in circle-shaped cell constructs at 20 µL/min microfluidic flow rate. A 3D co-culture increased the proliferation of BCOCs. We found that the appropriate time to evaluate the viability of BCOC, concentration of cytokines, and migration of monocytes was 6 h, 24 h, and three days after BGC treatment. Lastly, the immunotherapeutic effects of BCOC increased according to BCG dosage. To predict effects of immunotherapeutic agent in bladder cancer, we constructed a 3D bioprinted BCOC model. The BCOC was validated with BCG, which has been proven to be effective in the immunotherapy of bladder cancer. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Establishment of three-dimensional bioprinted bladder cancer-on-a-chip with a microfluidic system using bacillus calmette–guérin | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms22168887 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, v.22, no.16 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000690561000001 | - |
dc.identifier.scopusid | 2-s2.0-85112701969 | - |
dc.citation.number | 16 | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.volume | 22 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | 3D bioprinting | - |
dc.subject.keywordAuthor | BCG vaccine | - |
dc.subject.keywordAuthor | Intravesical administration | - |
dc.subject.keywordAuthor | Urinary bladder neoplasms | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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