Efficacy of Lactate-Utilizing Bacteria Isolated from the Rumen in Alleviating Subacute Ruminal Acidosis In VitroEfficacy of Lactate-Utilizing Bacteria Isolated from the Rumen in Alleviating Subacute Ruminal Acidosis In Vitro
- Authors
- Tabita Dameria Marbun; Kim, Gyeongjin; Park, Jinhyun; Kim, Chang-Hyun; Bae, Gui Seck; Chang, Moon Baek; Moon, Byeong Heoun; Yoon, Duhak; Lee, Sang Moo; Kim, Eun Joong
- Issue Date
- 2021
- Publisher
- 경상국립대학교 농업생명과학연구원
- Keywords
- in vitro; Rumen fermentation; Rumen lactate-utilizing bacteria; Subacute ruminal acidosis
- Citation
- 농업생명과학연구, v.55, no.3, pp 73 - 80
- Pages
- 8
- Journal Title
- 농업생명과학연구
- Volume
- 55
- Number
- 3
- Start Page
- 73
- End Page
- 80
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49727
- DOI
- 10.14397/jals.2021.55.3.73
- ISSN
- 1598-5504
2383-8272
- Abstract
- This study was conducted to evaluate the effect of rumen origin lactate-utilizing bacteria (LUB) as one of the potential treatments on subacute ruminal acidosis (SARA) during in vitro challenge compared to buffering agents (NaHCO3, sea minerals, MgO) and direct-fed microorganism (yeast). We hypothesized that rumen LUB (RLUB) could be a potential treatment to treat ruminal acidosis. The supplementation level of other treatments was determined by referring to previous studies in the literature. The 108 CFU/g freeze-dried RLUB isolated from Hanwoo cattle were compared with 0.1% NaHCO3, 0.8% of MgO, 0.5% sea mineral and 0.4% yeast during in vitro SARA challenge. Rumen fluid collected from one cannulated Holstein and one Hanwoo steer fed by maize silage was mixed with 0.5g feed consisted of 0.05g forage and 0.45g concentrate. These mixtures were incubated in triplication for 3, 6, 12 and 24h. After 6h of incubation, along with MgO and sea minerals, RLUB treatment showed higher (p<0.05) ㏗ values than control with no significant differences in total volatile fatty acid concentration. However, in the same period, the propionate concentration and A:P ratio were higher in RLUB than in the other treatment (p<0.05), which might alter the fermentation pattern. On the other hand, the RLUB treatment produced a higher (p<0.05) ammonia-N concentration. Based on these results, we can conclude that RLUB might have the potential to alleviate SARA. Nonetheless, further study on its mechanism in SARA is required, especially with live animals.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49727)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.