Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification
DC Field | Value | Language |
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dc.contributor.author | Kim, Jong-Gil | - |
dc.contributor.author | Baek, Seung Hoon | - |
dc.contributor.author | Kim, Seungrok | - |
dc.contributor.author | Kim, Hae In | - |
dc.contributor.author | Lee, Seung Woo | - |
dc.contributor.author | Le Minh Tu Phan | - |
dc.contributor.author | Kailasa, Suresh Kumar | - |
dc.contributor.author | Park, Tae Jung | - |
dc.date.available | 2019-01-22T11:37:25Z | - |
dc.date.issued | 2018-12 | - |
dc.identifier.issn | 0039-9140 | - |
dc.identifier.issn | 1873-3573 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/497 | - |
dc.description.abstract | Dengue virus (DENV) is one of the life-threatening viruses to the human. In this study, we have designed specific novel primers for rapid discriminative detection of DENV-1, DENV-2, and DENV-4 by real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction. The effect of parameters such as reaction temperature and magnesium sulfate was investigated on the RT-LAMP reaction for detection of DENV RNA. Under the optimal conditions, this method is able to differentiate and to detect DENV within 25 min, exhibiting detection limit of 3.5 copies/mu L. Importantly, the novel specific primers-based RT-LAMP assay did not react with other viruses, suggesting the selectivity of the method towards DENV RNA. The RT-LAMP reaction products are easily visualized with naked-eye when irradiated them under UV light at 365 nm. Amplification products could be visualized directly for color changes. This method provides a facile, and accurate molecular amplication technique for the rapid discriminative detection of dengue viruses. The RT-LAMP platform can be used as a promissing diagnostic tool for discriminative detection of DENV without aid of complicated protocols or sophisticated equipment. | - |
dc.format.extent | 6 | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.talanta.2018.08.019 | - |
dc.identifier.bibliographicCitation | TALANTA, v.190, pp 391 - 396 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000445306500050 | - |
dc.identifier.scopusid | 2-s2.0-85051393040 | - |
dc.citation.endPage | 396 | - |
dc.citation.startPage | 391 | - |
dc.citation.title | TALANTA | - |
dc.citation.volume | 190 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Dengue virus | - |
dc.subject.keywordAuthor | Specific primers | - |
dc.subject.keywordAuthor | Aedes aegypi mosquito | - |
dc.subject.keywordAuthor | Reverse trascription loop-mediated isothermal amplification | - |
dc.subject.keywordAuthor | Molecular diagnosis | - |
dc.subject.keywordPlus | AEDES-AEGYPTI DIPTERA | - |
dc.subject.keywordPlus | OF-CARE TESTS | - |
dc.subject.keywordPlus | RT-LAMP | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | SEROTYPES | - |
dc.subject.keywordPlus | NS1 | - |
dc.subject.keywordPlus | CULICIDAE | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | PCR | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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