Absolute oral and subcutaneous bioavailability of ortho-topolin riboside in mice
DC Field | Value | Language |
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dc.contributor.author | Won, J. | - |
dc.contributor.author | Oh, S.A. | - |
dc.contributor.author | Shin, H. | - |
dc.contributor.author | Kim, E. | - |
dc.contributor.author | Lee, G. | - |
dc.contributor.author | Noh, K. | - |
dc.contributor.author | Choi, H.-K. | - |
dc.contributor.author | Oh, S. | - |
dc.contributor.author | Kang, W. | - |
dc.date.accessioned | 2021-09-29T00:40:36Z | - |
dc.date.available | 2021-09-29T00:40:36Z | - |
dc.date.issued | 2021-11-30 | - |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.issn | 1873-264X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/49971 | - |
dc.description.abstract | Among essential phytohormones playing a pivotal role in regulating growth and development, ortho-topolin riboside (oTR) exerts the most substantial anti-tumor potency in various cancer cell lines. This study was designed to establish a quantitative determination method for oTR in mouse plasma using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), to validate the analytical method including stability, and to characterise its pharmacokinetic behaviour in mice. After simple protein precipitation with acetonitrile including kinetin riboside (internal standard), oTR was eluted on a reversed-phase column using a mobile phase of water and acetonitrile (3:7 v/v, including 0.1% formic acid). The protonated precursor ion [M+H]+ and major fragment ion were confirmed at m/z 374.06 and 241.99 for oTR, and 348.23 and 216.06 for the IS, respectively. oTR was stable under bench and storage conditions. The analytical method met the criteria of FDA-validated bioanalytical methods and was successfully applied to a pharmacokinetic study for the first time following oral, subcutaneous, and intravenous administrations. While oTR was merely absorbed by an oral route, 90% of the absolute subcutaneous bioavailability was observed. © 2021 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Absolute oral and subcutaneous bioavailability of ortho-topolin riboside in mice | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jpba.2021.114363 | - |
dc.identifier.bibliographicCitation | Journal of Pharmaceutical and Biomedical Analysis, v.206 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000701117100003 | - |
dc.identifier.scopusid | 2-s2.0-85114793751 | - |
dc.citation.title | Journal of Pharmaceutical and Biomedical Analysis | - |
dc.citation.volume | 206 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | HPLC-MS/MS | - |
dc.subject.keywordAuthor | Mice | - |
dc.subject.keywordAuthor | Ortho-topolin riboside | - |
dc.subject.keywordAuthor | Pharmacokinetics | - |
dc.subject.keywordPlus | CELL-LINE | - |
dc.subject.keywordPlus | KINETIN RIBOSIDE | - |
dc.subject.keywordPlus | HPLC-MS/MS | - |
dc.subject.keywordPlus | CYTOKININS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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