A method to monitor dutasteride in rat plasma using liquid-liquid extraction and multiple reaction monitoring: Comparisons and validation
DC Field | Value | Language |
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dc.contributor.author | Kang, M.J. | - |
dc.contributor.author | Cho, H.R. | - |
dc.contributor.author | Lee, D.H. | - |
dc.contributor.author | Yeom, D.W. | - |
dc.contributor.author | Choi, Y.W. | - |
dc.contributor.author | Choi, Y.S. | - |
dc.date.accessioned | 2021-09-30T01:40:25Z | - |
dc.date.available | 2021-09-30T01:40:25Z | - |
dc.date.issued | 2014-09-01 | - |
dc.identifier.issn | 2233-4203 | - |
dc.identifier.issn | 2093-8950 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50050 | - |
dc.description.abstract | Three different dutasteride extraction methods were compared and a method based on liquid-liquid extraction (LLE) using methyl tert-butyl ether and methylene chloride was proved to be more effective than others for the extraction of dutasteride and finasteride, the internal standard (IS), from rat plasma. Additionally, a method composed of the LLE extraction, liquid chromatography, and multiple reaction monitoring (MRM) to target dutasteride and IS was validated by assessing specificity, linearity (r2 = 0.9993, 5 - 400 ng/mL), sensitivity (the limit of detection: 4.03 ng/mL; the limit of quantitation: 12.10 ng/mL), accuracy (intra-day: 89.4 - 105.9%; inter-day: 84.9 - 100.9%), precision (intra-day: 0.8 - 6.9%; inter-day: 2.9 - 15.9%), and recovery (84.7 - 107.8%). Since the validated method was successfully applied to a pharmacokinetic study of dutasteride, it can be useful for the pharmacokinetic evaluation of newly developed dutasteride formulations. © 2014, Korean Society for Mass Spectrometry. All right reserved. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Korean Society for Mass Spectrometry | - |
dc.title | A method to monitor dutasteride in rat plasma using liquid-liquid extraction and multiple reaction monitoring: Comparisons and validation | - |
dc.type | Article | - |
dc.identifier.doi | 10.5478/MSL.2014.5.3.79 | - |
dc.identifier.bibliographicCitation | Mass Spectrometry Letters, v.5, no.3, pp 79 - 83 | - |
dc.identifier.kciid | ART001911817 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84907808559 | - |
dc.citation.endPage | 83 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 79 | - |
dc.citation.title | Mass Spectrometry Letters | - |
dc.citation.volume | 5 | - |
dc.type.docType | Letter | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Dutasteride | - |
dc.subject.keywordAuthor | Multiple reaction monitoring | - |
dc.subject.keywordAuthor | Rat plasma | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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