Validation of an HPLC/UV-based method for Salicornia herbacea-derived isorhamnetin-3-O-glucoside and quercetin-3-O-glucoside quantificationopen access
- Authors
- Park, Jun Yeon; Paje, Leo Adrianne; Kang, Ki Sung; Lee, Sanghyun
- Issue Date
- 2021
- Publisher
- 한국응용생명화학회
- Keywords
- Isorhamnetin-3-O-glucoside; Method validation; Quercetin-3-O-glucoside; Salicornia herbacea
- Citation
- Journal of Applied Biological Chemistry, v.64, no.3, pp 285 - 290
- Pages
- 6
- Journal Title
- Journal of Applied Biological Chemistry
- Volume
- 64
- Number
- 3
- Start Page
- 285
- End Page
- 290
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50084
- DOI
- 10.3839/jabc.2021.039
- ISSN
- 1976-0442
2234-7941
- Abstract
- Salicornia herbacea is a type of salt marsh plant that has been used in traditional medicine to treat several diseases.
Isorhamnetin-3-O-glucoside (I3G) and quercetin-3-O-glucoside (Q3G) are major flavonoids in S. herbacea that are known to exert various pharmacological activities. Therefore, our study sought to validate and optimize an HPLC/UV-based analytical method for I3G and Q3G yield quantification, as well as to determine its limit of detection, limit of quantification, linearity, precision, and accuracy. Upon testing a concentration range of 31.5-1.9 μg/mL the results exhibited good linearity (r2 ≥0.9996 and r2 ≥0.9999 for I3G and Q3G, respectively), and the procedure was deemed precise (relative standard deviation of ≤3.19 and ≤3.85%, respectively), and accurate (102.6-105.0 and 92.9-95.2%, respectively).
The results showed that our proposed method could be used for rapid I3G and Q3G evaluation in S. herbacea.
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