Improving osteogenesis activity on BMP-2-immobilized PCL fibers modified by the γ -ray irradiation technique
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yun, Y.-P. | - |
dc.contributor.author | Lee, J.Y. | - |
dc.contributor.author | Jeong, W.J. | - |
dc.contributor.author | Park, K. | - |
dc.contributor.author | Kim, H.-J. | - |
dc.contributor.author | Song, J.-J. | - |
dc.contributor.author | Kim, S.E. | - |
dc.contributor.author | Song, H.-R. | - |
dc.date.accessioned | 2021-10-13T03:40:31Z | - |
dc.date.available | 2021-10-13T03:40:31Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 2314-6133 | - |
dc.identifier.issn | 2314-6141 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50229 | - |
dc.description.abstract | The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the γ-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the γ-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP-2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and the mRNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells. © 2015 Young-Pil Yun et al. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Hindawi Publishing Corporation | - |
dc.title | Improving osteogenesis activity on BMP-2-immobilized PCL fibers modified by the γ -ray irradiation technique | - |
dc.type | Article | - |
dc.identifier.doi | 10.1155/2015/302820 | - |
dc.identifier.bibliographicCitation | BioMed Research International, v.2015 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84930654989 | - |
dc.citation.title | BioMed Research International | - |
dc.citation.volume | 2015 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordPlus | alkaline phosphatase | - |
dc.subject.keywordPlus | bone morphogenetic protein 2 | - |
dc.subject.keywordPlus | calcium | - |
dc.subject.keywordPlus | osteocalcin | - |
dc.subject.keywordPlus | osteopontin | - |
dc.subject.keywordPlus | polycaprolactone | - |
dc.subject.keywordPlus | alkaline phosphatase | - |
dc.subject.keywordPlus | BMP2 protein, human | - |
dc.subject.keywordPlus | bone morphogenetic protein 2 | - |
dc.subject.keywordPlus | polycaprolactone | - |
dc.subject.keywordPlus | polyester | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | bone development | - |
dc.subject.keywordPlus | cell differentiation | - |
dc.subject.keywordPlus | cell proliferation | - |
dc.subject.keywordPlus | contact angle | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | gamma radiation | - |
dc.subject.keywordPlus | gene expression | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | human tissue | - |
dc.subject.keywordPlus | hydrophilicity | - |
dc.subject.keywordPlus | scanning electron microscopy | - |
dc.subject.keywordPlus | X ray photoelectron spectroscopy | - |
dc.subject.keywordPlus | bone development | - |
dc.subject.keywordPlus | chemistry | - |
dc.subject.keywordPlus | gamma radiation | - |
dc.subject.keywordPlus | genetics | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | radiation response | - |
dc.subject.keywordPlus | Alkaline Phosphatase | - |
dc.subject.keywordPlus | Bone Morphogenetic Protein 2 | - |
dc.subject.keywordPlus | Cell Differentiation | - |
dc.subject.keywordPlus | Cell Proliferation | - |
dc.subject.keywordPlus | Gamma Rays | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Osteogenesis | - |
dc.subject.keywordPlus | Photoelectron Spectroscopy | - |
dc.subject.keywordPlus | Polyesters | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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