Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation
DC Field | Value | Language |
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dc.contributor.author | Choi, Mun Sun | - |
dc.contributor.author | Ze, Eun Young | - |
dc.contributor.author | Park, Jae Yong | - |
dc.contributor.author | Shin, Tae-Seop | - |
dc.contributor.author | Kim, Jae Gyu | - |
dc.date.accessioned | 2021-10-21T02:40:31Z | - |
dc.date.available | 2021-10-21T02:40:31Z | - |
dc.date.issued | 2021-07 | - |
dc.identifier.issn | 1226-3303 | - |
dc.identifier.issn | 2005-6648 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50476 | - |
dc.description.abstract | Background/Aims: Bacteria-derived outer membrane vesicles (OMVs) are commonly associated with various biological activities and functions. Helicobacter pylori- derived OMVs are thought to contribute to pathogenesis. This study aimed to investigate the effects of H. pylori-derived OMVs. Methods: H. pylori strains were isolated from patients with gastritis, gastric ulcer, or gastric cancer using endoscopic biopsy. The U-937, AGS, and MKN-45 cell lines were exposed to H. pylori and H. pylori-derived OMVs. The expression of interleukin 8 (IL-8) messenger RNA (mRNA) was assessed using reverse transcription- polymerase chain reaction (RT-PCR) and real-time RT-PCR, and IL-8 secretion was analyzed using enzyme-linked immunosorbent assay. Nuclear factor kappa B (NF-κB) activation was evaluated by Western blotting. Results: H. pylori and H. pylori-derived OMVs induced the expression of IL-8 mRNA and protein. Importantly, the bacteria induced higher IL-8 mRNA and protein expression than the OMVs. IL-8 expression was induced to different levels in response to H. pylori-derived OMVs from hosts with different gastric diseases. Western blotting revealed the increased phosphorylation and reduced degradation of inhibitor of NF-κB alpha in cells exposed to OMVs. Conclusions: H. pylori-derived OMVs may aid the development of various gastric diseases by inducing IL-8 production and NF-κB activation. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 대한내과학회 | - |
dc.title | Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation | - |
dc.type | Article | - |
dc.identifier.doi | 10.3904/kjim.2019.432 | - |
dc.identifier.bibliographicCitation | The Korean Journal of Internal Medicine, v.36, no.4, pp 857 - 867 | - |
dc.identifier.kciid | ART002726987 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000669004600015 | - |
dc.identifier.scopusid | 2-s2.0-85111558769 | - |
dc.citation.endPage | 867 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 857 | - |
dc.citation.title | The Korean Journal of Internal Medicine | - |
dc.citation.volume | 36 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Helicobacter pylori | - |
dc.subject.keywordAuthor | Extracellular vesicles | - |
dc.subject.keywordAuthor | Bacterial outer membrane proteins | - |
dc.subject.keywordAuthor | Interleukin-8 | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ULCER | - |
dc.subject.keywordPlus | IL-8 | - |
dc.subject.keywordPlus | AP-1 | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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