Evaluation of rapid SARS-CoV-2 antigen tests, AFIAS COVID-19 Ag and ichroma COVID-19 Ag, with serial nasopharyngeal specimens from COVID-19 patients
DC Field | Value | Language |
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dc.contributor.author | Kweon, Oh Joo | - |
dc.contributor.author | Lim, Yong Kwan | - |
dc.contributor.author | Kim, Hye Ryoun | - |
dc.contributor.author | Choi, Yoojeong | - |
dc.contributor.author | Kim, Min-Chul | - |
dc.contributor.author | Choi, Seong-Ho | - |
dc.contributor.author | Chung, Jin-Won | - |
dc.contributor.author | Lee, Mi-Kyung | - |
dc.date.accessioned | 2021-10-22T01:40:16Z | - |
dc.date.available | 2021-10-22T01:40:16Z | - |
dc.date.issued | 2021-04 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50527 | - |
dc.description.abstract | We evaluated the diagnostic accuracy of two newly developed, point-of-care, rapid antigen tests (RATs) for detecting SARS-CoV-2, the AFIAS COVID-19 Ag and the ichromaTM COVID-19 Ag, and investigated antigen kinetics. A total of 200 serially collected nasopharyngeal (NP) specimens from 38 COVID-19 patients and 122 specimens from negative controls were analyzed. Diagnostic sensitivity and specificity were assessed in comparison to molecular test results and subdivided according to targeted genes (E, RdRP, and N) and days post-symptom onset (PSO). For the kinetics evaluation, cut-off-indices from serial NP specimens were used according to the number of days PSO. Both RATs showed sensitivity of 91.3-100% for specimens with cycle threshold (Ct) < 25. The specificity of AFIAS was 98.7-98.9% and that of ichromaTM was 100.0%. The kappa values of AFIAS and ichromaTM for the molecular testing of specimens with Ct < 25 (RdRP) were 0.97 and 1.00, respectively. The sensitivity of AFIAS and ichromaTM for all genes was lower for specimens collected at 8-14 PSO than for those collected before 7-days PSO. The kinetics profiles showed that antigen levels gradually decreased from ≤ 7-days PSO to > 22-days PSO. Both RATs showed excellent specificity and acceptable sensitivity for NP specimens with higher viral loads and for specimens collected within 7-days PSO. Hence, they have the potential to become useful tools for the early detection of SARS-CoV-2. However, because of concerns about false negativity, RATs should be used in conjunction with molecular tests. Copyright: © 2021 Kweon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Public Library of Science | - |
dc.title | Evaluation of rapid SARS-CoV-2 antigen tests, AFIAS COVID-19 Ag and ichroma COVID-19 Ag, with serial nasopharyngeal specimens from COVID-19 patients | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0249972 | - |
dc.identifier.bibliographicCitation | PLoS ONE, v.16, no.4 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000639359600084 | - |
dc.identifier.scopusid | 2-s2.0-85104134133 | - |
dc.citation.number | 4 | - |
dc.citation.title | PLoS ONE | - |
dc.citation.volume | 16 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | virus antigen | - |
dc.subject.keywordPlus | virus RNA | - |
dc.subject.keywordPlus | adult | - |
dc.subject.keywordPlus | aged | - |
dc.subject.keywordPlus | antigen detection | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | clinical article | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | coronavirus disease 2019 | - |
dc.subject.keywordPlus | diagnostic accuracy | - |
dc.subject.keywordPlus | diagnostic test accuracy study | - |
dc.subject.keywordPlus | E gene | - |
dc.subject.keywordPlus | early diagnosis | - |
dc.subject.keywordPlus | false negative result | - |
dc.subject.keywordPlus | female | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | immunoaffinity chromatography | - |
dc.subject.keywordPlus | male | - |
dc.subject.keywordPlus | middle aged | - |
dc.subject.keywordPlus | N gene | - |
dc.subject.keywordPlus | nasopharyngeal swab | - |
dc.subject.keywordPlus | point of care testing | - |
dc.subject.keywordPlus | predictive value | - |
dc.subject.keywordPlus | RdRP gene | - |
dc.subject.keywordPlus | real time polymerase chain reaction | - |
dc.subject.keywordPlus | RNA extraction | - |
dc.subject.keywordPlus | sensitivity and specificity | - |
dc.subject.keywordPlus | Severe acute respiratory syndrome coronavirus 2 | - |
dc.subject.keywordPlus | symptom | - |
dc.subject.keywordPlus | time | - |
dc.subject.keywordPlus | very elderly | - |
dc.subject.keywordPlus | virus detection | - |
dc.subject.keywordPlus | virus gene | - |
dc.subject.keywordPlus | virus load | - |
dc.subject.keywordPlus | young adult | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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