Kordia aestuariivivens sp. nov. and Olleya sediminilitoris sp. nov., isolated from a tidal flat

Abstract

Two Gram-stain-negative and non-flagellated bacteria, YSTF-M3(T) and YSTF-M6(T), were isolated from a tidal flat from Yellow Sea, Republic of Korea, and subjected to a polyphasic taxonomic study. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strains YSTF-M3(T) and YSTF-M6(T) belong to the genera Kordia and Olleya of the family Flavobacteriaceae, respectively. The 16S rRNA gene sequence similarities between strain YSTF-M3(T) and the type strains of Kordia species and between strain YSTF-M6(T) and the type strains of Olleya species were 94.1-98.4 and 97.3-98.3%, respectively. The ANI and dDDH values between genomic sequences of strain YSTF-M3(T) and the type strains of five Kordia species and between those of strain YSTF-M6(T) and the type strains of three Olleya species were in ranges of 77.0-83.2 and 20.7-27.1% and 79.4-81.5 and 22.3-23.9%, respectively. The DNA G+C contents of strain YSTF-M3(T) and YSTF-M6(T) from genomic sequences were 34.1 and 31.1%, respectively. Both strains contained MK-6 as predominant menaquinone and phosphatidylethanolamine as only major phospholipid identified. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strains YSTF-M3(T) and YSTF-M6(T) are separated from recognized species of the genera Kordia and Olleya, respectively. On the basis of the data presented, strains YSTF-M3(T) (=KACC 21639T=NBRC 114499(T)) and YSTF-M6(T) (=KACC 21640(T)=NBRC 114500(T)) are considered to represent novel species of the genera Kordia and Olleya, respectively, for which the names Kordia aestuari-ivivens sp. nov. and Olleya sediminilitoris sp. nov. are proposed.