A sensitive analytical method for the determination of SG-SP1 in rat plasma by HPLC-MS/MS and its application to a pharmacokinetic study
DC Field | Value | Language |
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dc.contributor.author | Kang, J. | - |
dc.contributor.author | Won, J. | - |
dc.contributor.author | Fei, X. | - |
dc.contributor.author | Noh, K. | - |
dc.contributor.author | Kim, S.-H. | - |
dc.contributor.author | Seo, S.-Y. | - |
dc.contributor.author | Kang, W. | - |
dc.date.accessioned | 2021-10-29T01:40:13Z | - |
dc.date.available | 2021-10-29T01:40:13Z | - |
dc.date.issued | 2021-08-05 | - |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.issn | 1873-264X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/50894 | - |
dc.description.abstract | SG-SP1, a newly synthesised gallic acid derivative, blocks histamine release by reducing calcium influx in mast cells and inhibits inflammatory cytokine expression. This derivative has promising anti-allergic potential. Our research was designed to establish a quantitative determination method for SG-SP1 in rat plasma using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), to validate the analytical method including stability and to characterise its pharmacokinetic behaviour in rats. After simple protein precipitation with acetonitrile including an internal standard, SG-SP1 was eluted on a reversed-phase column using a mobile phase of water and acetonitrile (2:8 v/v, including 0.1 % formic acid). The protonated precursor ion [M+H]+ and major fragment ion were confirmed at m/z 588.2 and 180.1, respectively. The substance was stable under bench and storage conditions. The analytical method met the criteria of FDA-validated bioanalytical methods and was successfully applied to a pharmacokinetic study for the first time. SG-SP1 decayed in a biphasic pattern with terminal half-life of 5.1 h and clearance of about 3.2 L/h/kg. Double peaks were observed following oral administration, and the absolute oral bioavailability was ∼1 %. © 2021 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | A sensitive analytical method for the determination of SG-SP1 in rat plasma by HPLC-MS/MS and its application to a pharmacokinetic study | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jpba.2021.114151 | - |
dc.identifier.bibliographicCitation | Journal of Pharmaceutical and Biomedical Analysis, v.202 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000659231300009 | - |
dc.identifier.scopusid | 2-s2.0-85106401634 | - |
dc.citation.title | Journal of Pharmaceutical and Biomedical Analysis | - |
dc.citation.volume | 202 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | HPLC-MS/MS | - |
dc.subject.keywordAuthor | Pharmacokinetics | - |
dc.subject.keywordAuthor | Rats | - |
dc.subject.keywordAuthor | SG-SP1 | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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