Seed-Specifically Overexpressed Arabidopsis Cytochrome P450 85A2 Promotes Vegetative and Reproductive Growth and Development of Arabidopsis thaliana
DC Field | Value | Language |
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dc.contributor.author | Yeon, Myeong Hun | - |
dc.contributor.author | Park, Chan-Ho | - |
dc.contributor.author | Lee, Ye Eun | - |
dc.contributor.author | Roh, Jeehee | - |
dc.contributor.author | Kim, Seong-Ki | - |
dc.date.accessioned | 2021-11-19T07:40:10Z | - |
dc.date.available | 2021-11-19T07:40:10Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 1226-9239 | - |
dc.identifier.issn | 1867-0725 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51669 | - |
dc.description.abstract | Arabidopsis Cytochrome P450 85A2 (AtCYP85A2) was introduced to Arabidopsis thaliana seeds using a seed-specific promoter, pAt5g54000. GUS (beta-Glucuronidase) activity and RT-PCR analysis demonstrated that AtCYP85A2 overexpression only occurred in seeds of a transgenic plant, pAt5g54000-AtCYP85A2::Col-0. A crude enzyme solution prepared pAt5g54000-AtCYP85A2::Col-0 seeds successfully catalyzed the conversion of castasterone (CS) to brassinolide (BL), which was not detected in wild-type seeds. Furthermore, a higher level of CS and BL was detected in pAt5g54000-AtCYP85A2::Col-0 seeds compared to untransformed seeds, thus demonstrating that seed-specific overexpression of AtCYP85A2 efficiently encoded a bi-functional enzyme for brassinosteroids 6-oxidase/brassinolide synthase to generate CS and BL in seeds of pAt5g54000-AtCYP85A2::Col-0. Compared to the wild type, pAt5g54000-AtCYP85A2::Col-0 produced substantially larger seeds with a high concentration of nutrients due to an enhancement in brassinosteroids signaling. Additionally, pAt5g54000-AtCYP85A2::Col-0 exhibited superior seed germination, seedling and rosette plant growth, and flower and silique formation, indicating that seed-specific AtCYP85A2 expression activates overall vegetative and reproductive growth and development in A. thaliana. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | Seed-Specifically Overexpressed Arabidopsis Cytochrome P450 85A2 Promotes Vegetative and Reproductive Growth and Development of Arabidopsis thaliana | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s12374-021-09340-3 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PLANT BIOLOGY, v.65, no.1, pp 75 - 86 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000715649600001 | - |
dc.identifier.scopusid | 2-s2.0-85118581068 | - |
dc.citation.endPage | 86 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 75 | - |
dc.citation.title | JOURNAL OF PLANT BIOLOGY | - |
dc.citation.volume | 65 | - |
dc.type.docType | Article; Early Access | - |
dc.publisher.location | 독일 | - |
dc.subject.keywordAuthor | Arabidopsis thaliana | - |
dc.subject.keywordAuthor | Brassinosteroid | - |
dc.subject.keywordAuthor | Seed-specific expression | - |
dc.subject.keywordAuthor | Growth promotion | - |
dc.subject.keywordPlus | BRASSINOSTEROID SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | RECEPTOR KINASE | - |
dc.subject.keywordPlus | CASTASTERONE | - |
dc.subject.keywordPlus | BRASSINOLIDE | - |
dc.subject.keywordPlus | CYP85A2 | - |
dc.subject.keywordPlus | TOMATO | - |
dc.subject.keywordPlus | BRI1 | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.description.journalRegisteredClass | kciCandi | - |
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