Characterization and genome analysis of arthrobacter bangladeshi sp. Nov., applied for the green synthesis of silver nanoparticles and their antibacterial efficacy against drug-resistant human pathogens
DC Field | Value | Language |
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dc.contributor.author | Amdadul, Huq Md. | - |
dc.contributor.author | Akter, S. | - |
dc.date.accessioned | 2021-11-23T09:40:05Z | - |
dc.date.available | 2021-11-23T09:40:05Z | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.issn | 1999-4923 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51772 | - |
dc.description.abstract | The present study describes the isolation and characterization of novel bacterial species Arthrobacter bangladeshi sp. nov., applied for the green synthesis of AgNPs, and investigates its antibacterial efficacy against drug-resistant pathogenic Salmonella Typhimurium and Yersinia enterocolitica. Novel strain MAHUQ-56T is Gram-positive, aerobic, non-motile, and rod-shaped. Colonies were spherical and milky white. The strain showed positive activity for catalase and nitrate reductase, and the hydrolysis of starch, L-tyrosine, casein, and Tween 20. On the basis of the 16S rRNA gene sequence, strain MAHUQ-56T belongs to the Arthrobacter genus and is most closely elated to Arthrobacter pokkalii P3B162T (98.6%). Arthrobacter bangladeshi MAHUQ-56T has a genome 4,566,112 bp long (26 contigs) with 4125 protein-coding genes, 51 tRNA and 6 rRNA genes. The culture supernatant of Arthrobacter bangladeshi MAHUQ-56T was used for the easy and green synthesis of AgNPs. Synthesized AgNPs were characterized by UV–vis spectroscopy, FE-TEM, XRD, DLS, and FT-IR. Synthesized AgNPs were spherical and 12–50 nm in size. FT-IR analysis revealed various biomolecules that may be involved in the synthesis process. Synthesized AgNPs showed strong antibacterial activity against multidrug-resistant pathogenic S. typhimurium and Y. enterocolitica. MIC values of the synthesized AgNPs against S. typhimurium and Y. enterocolitica were 6.2 and 3.1 ug/mL, respectively. The MBC of synthesized AgNPs for both pathogens was 12.5 ug/mL. FE-SEM analysis revealed the morphological and structural alterations, and damage of pathogens treated by AgNPs. These changes might disturb normal cellular functions, which ultimately leads to the death of cells. © 2021 by the authors. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | Characterization and genome analysis of arthrobacter bangladeshi sp. Nov., applied for the green synthesis of silver nanoparticles and their antibacterial efficacy against drug-resistant human pathogens | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/pharmaceutics13101691 | - |
dc.identifier.bibliographicCitation | Pharmaceutics, v.13, no.10 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000716906100001 | - |
dc.identifier.scopusid | 2-s2.0-85118483545 | - |
dc.citation.number | 10 | - |
dc.citation.title | Pharmaceutics | - |
dc.citation.volume | 13 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | AgNPs | - |
dc.subject.keywordAuthor | Arthrobacter bangladeshiMAHUQ-56T | - |
dc.subject.keywordAuthor | Green synthesis | - |
dc.subject.keywordAuthor | S. typhimurium | - |
dc.subject.keywordAuthor | Y. enterocolitica | - |
dc.subject.keywordPlus | BIOLOGICAL SYNTHESIS | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | DATABASE | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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