Arenibacter arenosicollis sp. nov., isolated from a sand dune

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, designated BSSL-BM3T, was isolated from sand collected from a dune near the Yellow Sea, Republic of Korea, and subjected to a polyphasic taxonomic study. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BSSL-BM3T fell within the clade comprising the type strains of Arenibacter species. Strain BSSL-BM3T exhibited 16S rRNA gene sequence similarity values of 98.0–99.0% to the type strains of Arenibacter catalasegens, Arenibacter hampyeongensis, Arenibacter echinorum, Arenibacter palladensis and Arenibacter troitsensis and of 94.2–96.7% to the type strains of the other Arenibacter species. The averagenu-cleotide identity and digitalDNA–DNA hybridization values between strain BSSL-BM3T and the type strains of A. catalasegens, A. hampyeongensis, A. echinorum, A. palladensis and A. troitsensis were 82.2–88.8% and 25.0–36.5%, respectively. The DNA G+C content of strain BSSL-BM3T from genomic sequence data was 38.75 mol%. Strain BSSL-BM3T contained MK-6 as the predominant menaquinone and iso-C17:0 3-OH, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and iso-C15:1 G as the major fatty acids. The major polar lipids of strain BSSL-BM3T were phosphatidylethanolamine and two unidentified lipids. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain BSSL-BM3T is separated from recog-nized Arenibacter species. On the basis of the data presented here, strain BSSL-BM3T is considered to represent a novel species of the genus Arenibacter, for which the name Arenibacter arenosicollis sp. nov. is proposed. The type strain is BSSL-BM3T (=KACC 21632T=NBRC 114502T). © 2021 The Authors.