High concentration of sodium chloride could induce the viable and culturable states of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis
DC Field | Value | Language |
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dc.contributor.author | Song, Hana | - |
dc.contributor.author | Lee, Sun-Young | - |
dc.date.accessioned | 2021-11-26T08:40:12Z | - |
dc.date.available | 2021-11-26T08:40:12Z | - |
dc.date.issued | 2021-06 | - |
dc.identifier.issn | 0266-8254 | - |
dc.identifier.issn | 1472-765X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51917 | - |
dc.description.abstract | In the present study, Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis were transferred into Luria-Bertani medium without NaCl (LBWS) and adjusted to various pHs (4, 5, 6 and 7) with lactic acid containing 0 center dot 75, 5, 10 and 30% NaCl, and stored at 25 degrees C until the bacterial populations reached below detectable levels on tryptic soy agar (TSA). Although E. coli O157:H7 and S. Enteritidis did not grow on TSA when incubated in LBWS with 30% NaCl for 35 and 7 days, more than 60 and 70% of the bacterial cells were shown to be viable via fluorescent staining with SYTO9 and propidium iodide (PI), respectively, suggesting that a number of cells could be induced into the viable but nonculturable (VBNC) state. These bacteria that were induced into a VBNC state were transferred to a newly prepared tryptic soy broth (TSB) and then incubated at 37 degrees C for several days. After more than 7 days, E. coli O157:H7 and S. Enteritidis regained their culturability. We, therefore, suggest that E. coli O157:H7 and S. Enteritidis entered the VBNC state under the adverse condition of higher salt concentrations and were revived when these conditions were reversed. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | High concentration of sodium chloride could induce the viable and culturable states of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/lam.13468 | - |
dc.identifier.bibliographicCitation | LETTERS IN APPLIED MICROBIOLOGY, v.72, no.6, pp 741 - 749 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000625660000001 | - |
dc.identifier.scopusid | 2-s2.0-85102065859 | - |
dc.citation.endPage | 749 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 741 | - |
dc.citation.title | LETTERS IN APPLIED MICROBIOLOGY | - |
dc.citation.volume | 72 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Escherichia coli O157 | - |
dc.subject.keywordAuthor | H7 | - |
dc.subject.keywordAuthor | NaCl | - |
dc.subject.keywordAuthor | pH | - |
dc.subject.keywordAuthor | Salmonella enterica serovar Enteritidis | - |
dc.subject.keywordAuthor | survival | - |
dc.subject.keywordAuthor | VBNC | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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