N-Glycan Modifications with Negative Charge in a Natural Polymer Mucin from Bovine Submaxillary Glands, and Their Structural Role
DC Field | Value | Language |
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dc.contributor.author | Kim, Jihye | - |
dc.contributor.author | Lee, Byoungju | - |
dc.contributor.author | Lee, Junmyoung | - |
dc.contributor.author | Ji, Minkyoo | - |
dc.contributor.author | Park, Chi Soo | - |
dc.contributor.author | Lee, Jaeryong | - |
dc.contributor.author | Kang, Minju | - |
dc.contributor.author | Kim, Jeongeun | - |
dc.contributor.author | Jin, Mijung | - |
dc.contributor.author | Kim, Ha Hyung | - |
dc.date.accessioned | 2021-11-29T08:40:08Z | - |
dc.date.available | 2021-11-29T08:40:08Z | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/51989 | - |
dc.description.abstract | Bovine submaxillary mucin (BSM) is a natural polymer used in biomaterial applications for its viscoelasticity, lubricity, biocompatibility, and biodegradability. N-glycans are important for mucin stability and function, but their structures have not been fully characterized, unlike that of O-glycans. In this study, BSM N-glycans were investigated using liquid chromatography-tandem mass spectrometry. The microheterogeneous structures of 32 N-glycans were identified, and the quantities (%) of each N-glycan relative to total N-glycans (100%) were obtained. The terminal N-acetylgalactosamines in 12 N-glycans (sum of relative quantities; 27.9%) were modified with mono- (10 glycans) and disulfations (2 glycans). Total concentration of all sulfated N-glycans was 6.1 pmol in BSM (20 mu g), corresponding to 25.3% of all negatively charged glycans (sum of present N-glycans and reported O-glycans). No N-glycans with sialylated or phosphorylated forms were identified, and sulfate modification ions were the only negative charges in BSM N-glycans. Mucin structures, including sulfated N-glycans located in the hydrophobic terminal regions, were indicated. This is the first study to identify the structures and quantities of 12 sulfated N-glycans in natural mucins. These sulfations play important structural roles in hydration, viscoelasticity control, protection from bacterial sialidases, and polymer stabilization to support the functionality of BSM via electrostatic interactions. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI | - |
dc.title | N-Glycan Modifications with Negative Charge in a Natural Polymer Mucin from Bovine Submaxillary Glands, and Their Structural Role | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/polym13010103 | - |
dc.identifier.bibliographicCitation | POLYMERS, v.13, no.1 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000606266700001 | - |
dc.citation.number | 1 | - |
dc.citation.title | POLYMERS | - |
dc.citation.volume | 13 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | bovine submaxillary mucin | - |
dc.subject.keywordAuthor | N-glycan | - |
dc.subject.keywordAuthor | sulfate modification | - |
dc.subject.keywordAuthor | structural role | - |
dc.subject.keywordAuthor | functionality | - |
dc.subject.keywordPlus | SULFATED OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | LINKED GLYCANS | - |
dc.subject.keywordPlus | O-GLYCANS | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | FUCOSYLATION | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | STABILITY | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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