Cirsium japonicum var. maackii inhibits hydrogen peroxide-induced oxidative stress in SH-SY5Y cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김민정 | - |
dc.contributor.author | 이상현 | - |
dc.contributor.author | 김현영 | - |
dc.contributor.author | 조은주 | - |
dc.date.accessioned | 2021-12-01T00:40:20Z | - |
dc.date.available | 2021-12-01T00:40:20Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 2466-2402 | - |
dc.identifier.issn | 2466-2410 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/52095 | - |
dc.description.abstract | Over-produced reactive oxygen species (ROS) exert oxidative damage on lipids, proteins, and DNA in the human body, which leads to the onset of neurodegenerative diseases such as Alzheimer’s disease (AD). In this study, we explored the cellular antioxidant effect of Cirsium japonicum var. maackii (CJM) against hydrogen peroxide (H2O2)-induced oxidative stress in neuronal cells. The antioxidant activity was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, 2',7'-dichlorofluorescin diacetate and nitric oxide (NO) assays, and the molecular mechanisms were examined by Western blot analysis. H2O2 treatment of SH-SY5Y cells decreased cell viability and increased ROS and NO production compared to H2O2-untreated cells. However, CJM increased cell viability and decreased ROS and NO accumulation in the H2O2-treated SH-SY5Y cells compared to H2O2-treated control cells. Especially, the EtOAc fraction from CJM showed the strongest antioxidant effect compared with the other extracts and fractions. Therefore, we further examined the CJM mechanism against oxidative stress using the EtOAc fraction from CJM. The EtOAc fraction up-regulated the expressions of heme oxygenase-1, NAD(P)H quinone oxidoreductase 1, and thioredoxin reductase 1. These results indicate that CJM promotes the activation of antioxidative enzymes, which eliminate ROS and NO, and further leads to an increase in the cell viability. Taken together, our results show that CJM exhibited an antioxidant activity in H2O2-treated SH-SY5Y cells, and it could be a novel antioxidant agent for the prevention or treatment of neurodegenerative disease such as AD. | - |
dc.format.extent | 13 | - |
dc.language | 한국어 | - |
dc.language.iso | KOR | - |
dc.publisher | 충남대학교 농업과학연구소 | - |
dc.title | Cirsium japonicum var. maackii inhibits hydrogen peroxide-induced oxidative stress in SH-SY5Y cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.7744/kjoas.20210006 | - |
dc.identifier.bibliographicCitation | Korean Journal of Agricultural Science, v.48, no.1, pp 119 - 131 | - |
dc.identifier.kciid | ART002695395 | - |
dc.description.isOpenAccess | Y | - |
dc.citation.endPage | 131 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 119 | - |
dc.citation.title | Korean Journal of Agricultural Science | - |
dc.citation.volume | 48 | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Alzheimer’s disease | - |
dc.subject.keywordAuthor | Cirsium japonicum var. maackii | - |
dc.subject.keywordAuthor | hydrogen peroxide | - |
dc.subject.keywordAuthor | oxidative stress | - |
dc.subject.keywordAuthor | SH-SY5Y | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.