Antioxidant or apoptosis inhibitor supplementation in culture media improves post-thaw recovery of murine spermatogonial stem cells
DC Field | Value | Language |
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dc.contributor.author | Jung, Sang-Eun | - |
dc.contributor.author | Oh, Hui-Jo | - |
dc.contributor.author | Ahn, Jin-Seop | - |
dc.contributor.author | Kim, Yong-Hee | - |
dc.contributor.author | Kim, Bang-Jin | - |
dc.contributor.author | Ryu, Buom-Yong | - |
dc.date.accessioned | 2021-12-06T07:40:37Z | - |
dc.date.available | 2021-12-06T07:40:37Z | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 2076-3921 | - |
dc.identifier.issn | 2076-3921 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/52280 | - |
dc.description.abstract | We postulated that supplementation of antioxidant or apoptosis inhibitor in post-thaw culture media of spermatogonial stem cells (SSCs) alleviates reactive oxygen species (ROS) generation and apoptosis. Our aim was to develop an effective culture media for improving post-thaw recovery of SSCs. To determine the efficacy of supplementation with hypotaurine (HTU), α-tocopherol (α-TCP), and Z-DEVD-FMK (ZDF), we assessed the relative proliferation rate and SSC functional activity and performed a ROS generation assay, apoptosis assay, and western blotting for determination of the Bax/Bcl-xL ratio, as well as immunocytochemistry and real-time quantitative polymerase chain reaction (RT-qPCR) for SSC characterization. The relative proliferation rates with HTU 400 µM (133.7 ± 3.2%), α-TCP 400 µM (158.9 ± 3.6%), and ZDF 200 µM (133.1 ± 7.6%) supplementation were higher than that in the DMSO control (100 ± 3.6%). ROS generation was reduced with α-TCP 400 µM (0.8-fold) supplementation in comparison with the control (1.0-fold). Early apoptosis and Bax/Bcl-xL were lower with α-TCP 400 µM (2.4 ± 0.4% and 0.5-fold) and ZDF 200 µM (1.8 ± 0.4% and 0.3-fold) supplementation in comparison with the control (5.3 ± 1.4% and 1.0-fold) with normal characterization and functional activity. Supplementation of post-thaw culture media with α-TCP 400 µM and ZDF 200 µM improved post-thaw recovery of frozen SSCs via protection from ROS generation and apoptosis after cryo-thawing. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Antioxidant or apoptosis inhibitor supplementation in culture media improves post-thaw recovery of murine spermatogonial stem cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/antiox10050754 | - |
dc.identifier.bibliographicCitation | Antioxidants, v.10, no.5 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000653386700001 | - |
dc.identifier.scopusid | 2-s2.0-85105465427 | - |
dc.citation.number | 5 | - |
dc.citation.title | Antioxidants | - |
dc.citation.volume | 10 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | Antioxidant | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Permatogonial stem cells | - |
dc.subject.keywordAuthor | Post-thaw recovery | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
dc.subject.keywordPlus | Z-VAD-FMK | - |
dc.subject.keywordPlus | ALPHA-TOCOPHEROL | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | SPERM CHARACTERISTICS | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | CRYOPRESERVATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | HYPOTAURINE | - |
dc.subject.keywordPlus | VIABILITY | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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