Facile and foldable point-of-care biochip for nucleic acid based-colorimetric detection of murine norovirus in fecal samples using G-quadruplex and graphene oxide coated microbeads
DC Field | Value | Language |
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dc.contributor.author | Cui, W.Y. | - |
dc.contributor.author | Yoo, H.J. | - |
dc.contributor.author | Li, Y.G. | - |
dc.contributor.author | Baek, C. | - |
dc.contributor.author | Min, J. | - |
dc.date.accessioned | 2021-12-31T01:40:51Z | - |
dc.date.available | 2021-12-31T01:40:51Z | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.issn | 1873-4235 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/52810 | - |
dc.description.abstract | Norovirus is one of the most common causes of gastroenteritis, a disease characterized by diarrhea, vomiting, and stomach pain. A rapid on-site identification of the virus from fecal samples of patients is a prerequisite for accurate medical management. Here, we demonstrate a rapid nucleic acid-based detection platform as an on-site biosensing tool that can concentrate viruses from fecal samples. Moreover, it can perform RNA extraction and identification, and signal amplification using G-quadruplex and hemin containing DNA probes (G-DNA probes) and graphene oxide (GO)-coated microbeads. Briefly, murine noroviruses are lysed without chemicals on the surface of the GO microbeads. Subsequently, the target RNA is hybridized with G-DNA probes, and the resultant RNA/G-DNA probe complex is separated from unbound G-DNA probes using GO beads and is mixed with the detection buffer (ABTS/H2O2). Presence of murine noroviruses causes a colorimetric change of the buffer from colorless to green. Thus, we integrated all processes required to detect murine noroviruses in stool samples in a simple foldable microfluidic chip. Moreover, it can detect 101 pfu of the virus in 30 min in a fecal sample. © 2021 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Facile and foldable point-of-care biochip for nucleic acid based-colorimetric detection of murine norovirus in fecal samples using G-quadruplex and graphene oxide coated microbeads | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bios.2021.113878 | - |
dc.identifier.bibliographicCitation | Biosensors and Bioelectronics, v.199 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000773627800001 | - |
dc.identifier.scopusid | 2-s2.0-85121261095 | - |
dc.citation.title | Biosensors and Bioelectronics | - |
dc.citation.volume | 199 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Fecal samples | - |
dc.subject.keywordAuthor | Graphene oxide-coated microbeads | - |
dc.subject.keywordAuthor | Murine norovirus | - |
dc.subject.keywordAuthor | Nucleic-acid based detection | - |
dc.subject.keywordAuthor | On-site biosensor | - |
dc.subject.keywordAuthor | Sample preparation | - |
dc.subject.keywordPlus | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | ASYMPTOMATIC FOOD HANDLERS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | INFECTIONS | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | PCR | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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