Factors affecting penetration of zona-free hamster OVA
- Authors
- Chang, Y.S.; Lee, J.Y.; Moon, S.Y.; Kim, J.G.; Pang, M.G.; Shin, C.J.
- Issue Date
- 1990
- Publisher
- Informa Healthcare
- Keywords
- Acrosome reaction; Albumin; Capacitation; Sperm fertilizing capacity; Sperm penetration assay (SPA); Zona-free hamster eggs
- Citation
- Systems Biology in Reproductive Medicine, v.25, no.3, pp 213 - 224
- Pages
- 12
- Journal Title
- Systems Biology in Reproductive Medicine
- Volume
- 25
- Number
- 3
- Start Page
- 213
- End Page
- 224
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53544
- DOI
- 10.3109/01485019008987611
- ISSN
- 1939-6368
1939-6376
- Abstract
- The sperm penetration assay is an expensive, time-consuming test to assess male fertility in vitro. Although some investigators are enthusiastic in its application, others feel that it is not sensitive or specific enough to be used as part of the routine infertility evaluation. Indeed, this bioassay is not a faithful reproduction of in vivo conditions. However, if the SPA is abnormal, it is unlikely that sperm will fertilize a human ovum in vivo. Conversely, a normal SPA does not guarantee successful in vivo fertilization. No bioassay can be absolute in its predictive value, but false-negative results must be kept to a minimum for this bioassay to be of any clinical significance. Each laboratory performing the SPA should optimize the assay for sensitivity, reproducibility, and minimization of false-negative results and then establish normal and abnormal ranges of its own. If the limitations of the SPA are kept in mind, and if we employ it very selectively, it may still be useful. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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