Involvement of Mrs3/4 in Mitochondrial Iron Transport and Metabolism in Cryptococcus neoformans
DC Field | Value | Language |
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dc.contributor.author | Choi, Y. | - |
dc.contributor.author | Do, E. | - |
dc.contributor.author | Hu, G. | - |
dc.contributor.author | Caza, M. | - |
dc.contributor.author | Horianopoulos, L.C. | - |
dc.contributor.author | Kronstad, J.W. | - |
dc.contributor.author | Jung, W.H. | - |
dc.date.accessioned | 2022-01-17T02:44:02Z | - |
dc.date.available | 2022-01-17T02:44:02Z | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53631 | - |
dc.description.abstract | Mitochondria play a vital role in iron uptake and metabolism in pathogenic fungi, and also influence virulence and drug tolerance. However, the regulation of iron transport within the mitochondria of Cryptococcus neoformans, a causative agent of fungal meningoencephalitis in immunocompromised individuals, remains largely uncharacterized. In this study, we identified and functionally characterized Mrs3/4, a homolog of the Saccharomyces cerevisiae mitochondrial iron transporter, in C. neoformans var. grubii. A strain expressing an Mrs3/4-GFP fusion protein was generated, and the mitochondrial localization of the fusion protein was confirmed. Moreover, a mutant lacking the MRS3/4 gene was constructed; this mutant displayed significantly reduced mitochondrial iron and cellular heme accumulation. In addition, impaired mitochondrial iron-sulfur cluster metabolism and altered expression of genes required for iron uptake at the plasma membrane were observed in the mrs3/4 mutant, suggesting that Mrs3/4 is involved in iron import and metabolism in the mitochondria of C. neoformans. Using a murine model of cryptococcosis, we demonstrated that an mrs3/4 mutant is defective in survival and virulence. Taken together, our study suggests that Mrs3/4 is responsible for iron import in mitochondria and reveals a link between mitochondrial iron metabolism and the virulence of C. neoformans. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NLM (Medline) | - |
dc.title | Involvement of Mrs3/4 in Mitochondrial Iron Transport and Metabolism in Cryptococcus neoformans | - |
dc.type | Article | - |
dc.identifier.doi | 10.4014/jmb.2004.04041 | - |
dc.identifier.bibliographicCitation | Journal of microbiology and biotechnology, v.30, no.8, pp 1142 - 1148 | - |
dc.identifier.kciid | ART002618414 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000577538800005 | - |
dc.identifier.scopusid | 2-s2.0-85090077717 | - |
dc.citation.endPage | 1148 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1142 | - |
dc.citation.title | Journal of microbiology and biotechnology | - |
dc.citation.volume | 30 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | Cryptococcus neoformans | - |
dc.subject.keywordAuthor | iron | - |
dc.subject.keywordAuthor | iron transport | - |
dc.subject.keywordAuthor | mitochondria | - |
dc.subject.keywordAuthor | Mrs3/4 | - |
dc.subject.keywordPlus | CARRIER PROTEINS | - |
dc.subject.keywordPlus | VIRULENCE | - |
dc.subject.keywordPlus | ACQUISITION | - |
dc.subject.keywordPlus | TRAFFICKING | - |
dc.subject.keywordPlus | BIOGENESIS | - |
dc.subject.keywordPlus | MATURATION | - |
dc.subject.keywordPlus | FRATAXIN | - |
dc.subject.keywordPlus | PLAYS | - |
dc.subject.keywordPlus | MRS4 | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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