Anti-inflammatory effect of neo-lignan isoamericanin A via suppression of NF-kappa B in liposaccharide-stimulated RAW 264.7 cells
DC Field | Value | Language |
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dc.contributor.author | Yeo, Ji Yun | - |
dc.contributor.author | Hwang, Kwang Woo | - |
dc.contributor.author | Park, So-Young | - |
dc.date.accessioned | 2022-01-18T03:40:15Z | - |
dc.date.available | 2022-01-18T03:40:15Z | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 1596-5996 | - |
dc.identifier.issn | 1596-9827 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53703 | - |
dc.description.abstract | Purpose: To investigate the potential anti-inflammatory effects of the seeds of Opuntina humifusa and its active constituents. Methods: The extract of O. humifusa seeds was tested for the inhibition of nitric oxide (NO) production in liposaccharide (LPS)-stimulated RAW 264.7 cells using Griess reagent. The active constituents were isolated using bioassay-guided isolation methods. The effects of the active constituent on NO, proinflammatory cytokines, nuclear factor-kappa B (NF-kappa B) and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor (I kappa B) were evaluated by enzyme-linked immunosorbent assay (ELISA) and western blot analysis. Results: The seed extract of O. humifusa significantly attenuated LPS-induced NO production in RAW 264.7 cells (p < 0.05). Bioassay-guided fractionation resulted in the isolation of isoamericanin A as an active constituent. Isoamericanin A reduced LPS-induced production of NO, iNOS, and proinflammatory cytokines (TNF-alpha and IL-6) in a concentration-dependent manner (p < 0.05). Furthermore, the effect was accompanied by decreased translocation of NF-kappa B from the cytosol to the nucleus and the decreased phosphorylation of I kappa B in the cytosol induced by LPS (p < 0.05). Conclusion: The seed extract of O. humifusa and its active constituent, isoamericanin A, have antiinflammatory effects in LPS-stimulated RAW 264.7 cells, suggesting that they have potentials as antiinflammatory agents. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PHARMACOTHERAPY GROUP | - |
dc.title | Anti-inflammatory effect of neo-lignan isoamericanin A via suppression of NF-kappa B in liposaccharide-stimulated RAW 264.7 cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.4314/tjpr.v19i9.9 | - |
dc.identifier.bibliographicCitation | TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, v.19, no.9, pp 1857 - 1862 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000577194400009 | - |
dc.identifier.scopusid | 2-s2.0-85095406794 | - |
dc.citation.endPage | 1862 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1857 | - |
dc.citation.title | TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | - |
dc.citation.volume | 19 | - |
dc.type.docType | Article | - |
dc.publisher.location | 나이지리아 | - |
dc.subject.keywordAuthor | Opuntia humifusa seeds | - |
dc.subject.keywordAuthor | Isoamericanin A | - |
dc.subject.keywordAuthor | Nitric oxide | - |
dc.subject.keywordAuthor | RAW 264.7 cells | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordPlus | OPUNTIA-HUMIFUSA | - |
dc.subject.keywordPlus | SEEDS | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | EXTRACTS | - |
dc.subject.keywordPlus | STRESS | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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