Recombinant Histidine-Tagged Nano-protein-based Highly Sensitive Electro-Sensing Device for Salivary Cortisol
DC Field | Value | Language |
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dc.contributor.author | Chavan, Sachin Ganpat | - |
dc.contributor.author | Yagati, Ajay Kumar | - |
dc.contributor.author | Koyappayil, Aneesh | - |
dc.contributor.author | Go, Anna | - |
dc.contributor.author | Yeon, Sangho | - |
dc.contributor.author | Lee, Min-Ho | - |
dc.date.accessioned | 2022-01-20T06:40:38Z | - |
dc.date.available | 2022-01-20T06:40:38Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 1567-5394 | - |
dc.identifier.issn | 1878-562X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/53993 | - |
dc.description.abstract | We have developed a powerful biosensing strategy for immobilizing histidine-tagged (His-Tag)-oriented recombinant nano-protein immobilization on a chemically modified glassy carbon electrode (GCE) surfaces via (S)-N-(5-amino-1-carboxypentyl)iminodiacetic acid (ANTA) acting as a chelating Ni2+ centered interaction. Here, we introduce a label-free electro-sensor to quantify cortisol levels in saliva samples for point-of-care testing (POCT). The high specificity of the chemically modified GCE was established by genetically bio-engineered metal-binding sites on the selected recombinant apoferritin (R-AFTN) nano-protein to impart functionality to its surface and by coating the carbon surface with the self-assembled monolayers of 4-aminobenzoic acid (4-ABA) attached to ANTA groups complexed with Ni2+ transition metal ions. Despite the variety of conventional assays available to monitor cortisol levels, they require bulky exterior outfits, which hinders use in the healthcare systems. Therefore, we performed a rapid, easy-to-implement, and low-cost quantitative electro-sensor to enable the real-time detection of cortisol levels in saliva samples. As a result, the cortisol electro-sensor fabricated with high specificity utilizing a GCE could measure cortisol levels with a detection limit of 0.95 ng/ml and sensitivity of 7.91 μA/(ng/mL), which is a practical approach in human saliva. Thus, protein nanoprobe-based cortisol biosensing showed high sensitivity and selectivity for the direct electro-sensing of cortisol for POCT. © 2021 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Recombinant Histidine-Tagged Nano-protein-based Highly Sensitive Electro-Sensing Device for Salivary Cortisol | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bioelechem.2021.108046 | - |
dc.identifier.bibliographicCitation | Bioelectrochemistry, v.144 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000790445700003 | - |
dc.identifier.scopusid | 2-s2.0-85122561720 | - |
dc.citation.title | Bioelectrochemistry | - |
dc.citation.volume | 144 | - |
dc.type.docType | Article | - |
dc.publisher.location | 스위스 | - |
dc.subject.keywordAuthor | (S)-N-(5-Amino-1-carboxypentyl)iminodiacetic acid (ANTA) | - |
dc.subject.keywordAuthor | Antigen-antibody reaction | - |
dc.subject.keywordAuthor | Electrophysiological cortisol sensor | - |
dc.subject.keywordAuthor | Glassy carbon electrode surface modification | - |
dc.subject.keywordAuthor | R-AFTN nanoprobe | - |
dc.subject.keywordAuthor | Salivary stress testing | - |
dc.subject.keywordPlus | CARBON ELECTRODE | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | APOFERRITIN | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | ASSAY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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