In vivo time-dependent gene expression of cationic lipid-based emulsion as a stable and biocompatible non-viral gene carrier
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Seok Min | - |
dc.contributor.author | Nam, Hae Yun | - |
dc.contributor.author | Nam, Taehwan | - |
dc.contributor.author | Park, Kyeongsoon | - |
dc.contributor.author | Lee, Seulki | - |
dc.contributor.author | Kim, Kwangmeyung | - |
dc.contributor.author | Kwon, Ick Chan | - |
dc.contributor.author | Kim, Jun | - |
dc.contributor.author | Kang, Dongmin | - |
dc.contributor.author | Park, Jae Hyung | - |
dc.contributor.author | Jeong, Seo Young | - |
dc.date.accessioned | 2022-01-25T01:42:11Z | - |
dc.date.available | 2022-01-25T01:42:11Z | - |
dc.date.issued | 2008-05 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.issn | 1873-4995 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/54299 | - |
dc.description.abstract | To make stable and biocompatible non-viral gene carriers for therapeutic gene therapy, we developed a cationic lipid-based emulsion (CLE) prepared by an oil-in-water (O/W) emulsion method, wherein squalene oil was used as an oil core and the cationic lipid, 1,2-dioleoyl-sn-glycero-3-trimethylammonium-propane (DOTAP), was employed as an emulsifier. To evaluate in vivo characteristics such as toxicity and time-dependent gene expression, a bioluminescence reporter gene in pCMV-luc plasmid DNA was simply mixed with CLE in aqueous condition, resulting in a CLE/DNA complex. The CLE/DNA complex was optimized to form a compact and stable nano-sized particle by adding different amounts of plasmid DNA, and an optimal cationic lipid-to-DNA (C/D) weight ratio of 4 was identified. Freshly prepared CLE/DNA complex, with a C/D of 4, showed a high transfection efficiency and minimal cytotoxicity in vitro, compared to controls of a liposome (DOTAP)/DNA complex and a branched poly(ethyleneimine) (Mw=25 kDa) (bPEI)/DNA complex, respectively. The in vivo characteristics of the CLE/DNA complex were evaluated after intravenous injection into Balb/c mice. Time-dependent gene expression data in vivo were obtained using a non-invasive, whole animal bioluminescence imaging system. These data showed that the CLE/DNA complex offered prolonged high-level gene expression for I week, particularly in the liver and spleen. On the other hand, the controls of DOTAP/DNA complex and bPEI/DNA complex showed a relatively lower gene expression, because of the unstable and toxic properties of the control carriers. Our in vivo gene expression data demonstrate the potential of the CLE/DNA complex as a non-viral gene carrier for in vivo gene delivery. (C) 2008 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | In vivo time-dependent gene expression of cationic lipid-based emulsion as a stable and biocompatible non-viral gene carrier | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jconrel.2008.02.004 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.128, no.1, pp 89 - 97 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000256852100009 | - |
dc.citation.endPage | 97 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 89 | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 128 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | cationic lipid-based emulsions | - |
dc.subject.keywordAuthor | non-viral gene carrier | - |
dc.subject.keywordAuthor | in vivo toxicity | - |
dc.subject.keywordAuthor | in vivo gene expression | - |
dc.subject.keywordAuthor | bioluminescent imaging | - |
dc.subject.keywordPlus | NONIONIC SURFACTANTS | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | LIPOSOMES | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | VITRO | - |
dc.subject.keywordPlus | STABILITY | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.