Anti-amyloidogenic effect of menaquinone-7 on beta-amyloid production and aggregation
DC Field | Value | Language |
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dc.contributor.author | Lee, Chung-Hyun | - |
dc.contributor.author | Jo, Eun-Sang | - |
dc.contributor.author | Yeo, Ji-Yun | - |
dc.contributor.author | Choi, Seong Jun | - |
dc.contributor.author | Hwang, Kwang Woo | - |
dc.contributor.author | Park, So-Young | - |
dc.date.accessioned | 2022-02-17T03:41:25Z | - |
dc.date.available | 2022-02-17T03:41:25Z | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 1596-5996 | - |
dc.identifier.issn | 1596-9827 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/55110 | - |
dc.description.abstract | Purpose: To investigate the beneficial effects of menaquinone-7 (MK-7), an isoform of vitamin K-2, against beta-amyloid (A beta) production and aggregation in Alzheimer's disease using in vitro assays. Methods: The cytotoxicity of MK-7 was determined by MTT assay. The amount of A beta produced and secreted into the supernatant by APP-CHO cells treated with MK-7 was evaluated by ELISA. The expression of beta-secretases and ADAM10, a representative alpha-secretase, was determined using western blot analysis. The production of sAPP beta and sAPP alpha fragments generated by beta-secretases and alpha-secretase, respectively, were also determined by western blot analysis. The effect on A beta aggregation was assessed using Thioflavin T (Th T) assay. Results: MK-7 (up to 75 nM) significantly decreased A beta production in APP-CHO cells. This was accompanied by decreased expression of beta-secretase and lower production of sAPP beta (p < 0.05). However, expression of ADAM10 and production of sAPP alpha were not significantly affected. In contrast, MK-7 significantly decreased A beta aggregation in a dose-dependent manner (p < 0.05). Conclusion: MK-7 exerts anti-amyloidogenic effects via decreased production and lower aggregation of A beta into oligomers and fibrils. Therefore, dietary supplementation with MK-7 may be beneficial for the prevention of Alzheimer's disease. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PHARMACOTHERAPY GROUP | - |
dc.title | Anti-amyloidogenic effect of menaquinone-7 on beta-amyloid production and aggregation | - |
dc.type | Article | - |
dc.identifier.doi | 10.4314/tjpr.v21i1.15 | - |
dc.identifier.bibliographicCitation | TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, v.21, no.1, pp 93 - 97 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000752051200004 | - |
dc.identifier.scopusid | 2-s2.0-85124625661 | - |
dc.citation.endPage | 97 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 93 | - |
dc.citation.title | TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | - |
dc.citation.volume | 21 | - |
dc.type.docType | Article | - |
dc.publisher.location | 나이지리아 | - |
dc.subject.keywordAuthor | Menaquinone-7 | - |
dc.subject.keywordAuthor | Beta-amyloid | - |
dc.subject.keywordAuthor | beta-Secretases | - |
dc.subject.keywordAuthor | Aggregation | - |
dc.subject.keywordAuthor | Alzheimer's disease | - |
dc.subject.keywordAuthor | Vitamin K-2 | - |
dc.subject.keywordPlus | VITAMIN-K | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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